Characterization and Functional Analysis of PEBP Family Genes in Upland Cotton (Gossypium hirsutum L.).
Characterization and Functional Analysis of PEBP Family Genes in Upland Cotton (Gossypium hirsutum L.).
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DOI：
10.1371/journal.pone.0161080
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Yu S
中科院分区：
文献类型：
--
作者：
Zhang X;Wang C;Pang C;Wei H;Wang H;Song M;Fan S;Yu S
高地棉（Gossypium hirsutum L.）是一种天然存在的光周期敏感的多年生植物物种。然而，在驯化过程中失去了对日长的敏感性。磷脂酰乙醇胺结合蛋白（PEBP）基因家族在被子植物中具有促进和抑制开花的光周期途径，目前已鉴定出3个亚支。最近的证据表明PEBP家族基因在产生移动的开花信号中起重要作用。我们分离了高地棉PEBP基因家族的同源物，并研究了它们的调控和功能。克隆了9个PEBP样基因，系统发育分析表明，它们分属于FT、MFT、TFL 1和PEBP 4个亚支。棉花PEBP类基因在不同基因型、光周期反应和品种成熟度下表现出不同的表达模式。半野生型高地棉GhFT基因在短日照条件下表达量明显高于野生型，而GhPEBP 2基因在长日照条件下表达量明显高于野生型。我们还阐明，GhFT，而不是GhPEBP 2与FD-样bZIP转录因子GhFD相互作用，促进开花在长日照和短日照条件下。本研究结果表明，GhPEBP样基因可能执行不同的功能。本研究证实了PEBP类基因参与不同基因型棉花的光周期反应和开花时间调控，有助于进一步了解PEBP类基因在棉花中的功能。
Upland cotton (Gossypium hirsutum L.) is a naturally occurring photoperiod-sensitive perennial plant species. However, sensitivity to the day length was lost during domestication. The phosphatidylethanolamine-binding protein (PEBP) gene family, of which three subclades have been identified in angiosperms, functions to promote and suppress flowering in photoperiod pathway. Recent evidence indicates that PEBP family genes play an important role in generating mobile flowering signals. We isolated homologues of the PEBP gene family in upland cotton and examined their regulation and function. Nine PEBP-like genes were cloned and phylogenetic analysis indicated the genes belonged to four subclades (FT, MFT, TFL1 and PEBP). Cotton PEBP-like genes showed distinct expression patterns in relation to different cotton genotypes, photoperiod responsive and cultivar maturity. The GhFT gene expression of a semi-wild race of upland cotton were strongly induced under short day condition, whereas the GhPEBP2 gene expression was induced under long days. We also elucidated that GhFT but not GhPEBP2 interacted with FD-like bZIP transcription factor GhFD and promote flowering under both long- and short-day conditions. The present result indicated that GhPEBP-like genes may perform different functions. This work corroborates the involvement of PEBP-like genes in photoperiod response and regulation of flowering time in different cotton genotypes, and contributes to an improved understanding of the function of PEBP-like genes in cotton.