The sHSP22 Heat Shock Protein Requires the ABI1 Protein Phosphatase to Modulate Polar Auxin Transport and Downstream Responses
The sHSP22 Heat Shock Protein Requires the ABI1 Protein Phosphatase to Modulate Polar Auxin Transport and Downstream Responses
复制标题
sHSP22 热休克蛋白需要 ABI1 蛋白磷酸酶来调节极性生长素转运和下游反应
DOI：
10.1104/pp.17.01206
复制
发表时间：
2018-03-01
期刊：
影响因子：
7.4
通讯作者：
Xie, Qi
中科院分区：
文献类型：
--
作者：
Li, Yanli;Li, Yaqiong;Xie, Qi
植物激素脱落酸（阿坝）和吲哚乙酸（IAA）反应途径通过协同或拮抗作用调节植物的发育和环境适应。在这里，我们表明，ABI 1，一个关键的负调节阿坝信号，是必不可少的生长素调制的根的发展。我们进行了微阵列分析，使用功能丧失突变体abi 1 -3和Col-0幼苗用IAA处理。对于内质网（ER）小热休克蛋白编码基因sHSP 22，IAA的诱导依赖于ABI 1。shsp 22对阿坝的敏感性增强。相反，过表达全长，但不是截短的sHSP 22缺乏信号肽和ER保留序列，导致阿坝敏感性下降。过表达（OX）的sHSP 22部分地挽救了abi 1 -3的阿坝超敏性。此外，sHSP 22还参与了生长素调控的高温下胚轴伸长过程。sHSP 22也影响生长素外排载体PIN蛋白的积累，由于增强细胞内运输。sHSP 22 OX株系在生长素处理后侧根发生增多。我们的研究结果表明，sHSP 22通过调节生长素极性运输来调节生长素反应，ABI 1-sHSP 22提供了一个新的调控阿坝和生长素信号串扰的模块。
The phytohormones abscisic acid (ABA) and indole-3-acetic acid (IAA) response pathways interact synergistically or antagonistically to regulate plant development and environmental adaptation. Here, we show that ABI1, a key negative regulator of ABA signaling, is essential for auxin-modulated root development. We performed a microarray analysis using the loss-of-function mutant abi1-3 and Col-0 seedlings treated with IAA. For sHSP22, an endoplasmic reticulum (ER) small heat shock protein-encoding gene, the induction by IAA was dependent on ABI1. shsp22 displayed enhanced sensitivity to ABA in primary root growth. In contrast, overexpression of full-length, but not truncated sHSP22 lacking signal peptide and ER-retention sequences, resulted in decreased ABA sensitivity. Overexpressed (OX) sHSP22 partially rescued the ABA hypersensitivity of abi1-3. In addition, sHSP22 is involved in auxin-regulated hypocotyl elongation at high temperature treatment. sHSP22 also affected accumulation of auxin efflux carrier PIN proteins due to potentiated intracellular trafficking. And sHSP22 OX lines initiated more lateral roots after auxin application. Our results suggest that sHSP22 regulates auxin response through modulating auxin polar transport, and ABI1-sHSP22 provides a novel module orchestrating ABA and auxin signaling crosstalk in Arabidopsis (Arabidopsis thaliana).