Expression of Hypoxia-inducible Factor-1α, Vascular Endothelial Growth Factor, and Matrix Metalloproteinases 1, 3, and 9 in Hypertrophied Ligamentum Flavum
Expression of Hypoxia-inducible Factor-1α, Vascular Endothelial Growth Factor, and Matrix Metalloproteinases 1, 3, and 9 in Hypertrophied Ligamentum Flavum
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DOI：
10.1097/bsd.0b013e3182495b88
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发表时间：
2013-10-01
影响因子：
--
通讯作者：
Foelsch, Christian
中科院分区：
文献类型：
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作者：
Lakemeier, Stefan;Schofer, Markus D.;Foelsch, Christian
研究设计：免疫组织学研究目的：阐明基质金属蛋白酶（MMPs）、缺氧诱导因子-1（HIF）和血管内皮生长因子（VEGF）在从腰椎管狭窄症（LSS.Summary of Background Data）患者中获得的肥大黄韧带（LF）中的作用：老年人最常见的脊柱疾病是LSS，其部分原因是LF肥大。虽然以前的组织学和免疫化学研究已经在这方面进行，失去弹性和肥大的病理生理学是不完全understood.Methods：LF样品的38例LSS在脊髓减压过程中收获。12个LF样本来自椎间盘突出患者，术前磁共振成像上无可见变性，作为对照。将样品脱水并石蜡包埋。对于VEGF、HIF和MMPs 1、3和9表达的免疫组织化学测定，切片用VEGF、HIF和MMP抗体稀释液染色。Masson-Goldner染色后计数新生血管密度和弹性纤维数。结果：肥大组LF中MMPs 1、3、9和VEGF表达均明显增加（P
Study Design:Immunohistological study.Objective:To elucidate the role of matrix metalloproteinases (MMPs), hypoxia-inducible factor-1 (HIF), and vascular endothelial growth factor (VEGF) in the hypertrophied ligamentum flavum (LF) obtained from patients with lumbar spinal stenosis (LSS).Summary of Background Data:The most common spinal disorder in the elderly is LSS, which results in part from LF hypertrophy. Although prior histologic and immunochemical studies have been performed in this area, the pathophysiology of loss of elasticity and hypertrophy is not completely understood.Methods:LF samples of 38 patients with LSS were harvested during spinal decompression. Twelve LF samples obtained from patients with disk herniation and no visible degeneration on preoperative magnetic resonance imaging were obtained as controls. Samples were dehydrated and paraffin embedded. For immunohistochemical determination of VEGF, HIF, and MMPs 1, 3, and 9 expression, slices were stained with VEGF, HIF, and MMP antibody dilution. Neovessel density and number of elastic fibers were counted after Masson-Goldner staining. LF hypertrophy and cross-sectional area (CSA) were measured on T1-weighted magnetic resonance imaging.Results:MMPs 1, 3, 9 and VEGF expression were significantly increased in the hypertrophy group (P