A protocol for digesting internal soft tissues and mounting spiders for scanning electron microscopy
A protocol for digesting internal soft tissues and mounting spiders for scanning electron microscopy
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DOI：
10.1636/sh06-55.1
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发表时间：
2007-01-01
影响因子：
1
通讯作者：
Hormiga, Gustavo
中科院分区：
文献类型：
--
作者：
Alvarez-Padilla, Fernando;Hormiga, Gustavo
我们描述了一种使用一种称为胰酶的酶复合体来消化蜘蛛内部软组织的简单方案。这项技术比用腐蚀剂消化更受欢迎，因为它更好地保存了角质层表面，允许通过扫描电子或透射式光学显微镜进行研究。此外，我们描述了一种使用丙烯聚合物安装扫描电子显微镜蜘蛛身体部位的技术。
We describe a simple protocol for digesting the internal soft tissues of spiders using an enzyme complex known as pancreatin. This technique is preferred over digestions with caustic agents because it better preserves the cuticle surface, allowing its study by means of scanning electron or transmitted light microscopy. In addition, we describe a technique for mounting spider body parts for scanning electron microscopy using an acryloid polymer.