Blastomonas marina sp. nov., a bacteriochlorophyll-containing bacterium isolated from seawater.
Blastomonas marina sp. nov., a bacteriochlorophyll-containing bacterium isolated from seawater.
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DOI：
10.1099/ijsem.0.002070
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发表时间：
2017
影响因子：
2.8
通讯作者：
Yu-Chen Meng;Hong-Can Liu;YING-QIAN Kang;Yuguang Zhou;M. Cai
中科院分区：
文献类型：
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作者：
Yu-Chen Meng;Hong-Can Liu;YING-QIAN Kang;Yuguang Zhou;M. Cai
从东海沿海水域分离出革兰氏染色阴性、兼性厌氧、深黄色色素细菌SSR2A-4-2T。细胞呈卵球形或短杆状，有糙皮鞭毛，除含有细菌叶绿素a色素外，还含有类胡萝卜素。基于16S rRNA基因序列的系统发育树图显示，菌株SSR2A-4-2T与blastomasnatatoria EY 4220T (=DSM 3183T)（相似度95.6%）、Blastomonasursincola pr - 99t (=DSM 9006T)（相似度95.5 %）和Blastomonasaquatica PE4-5T (=JCM 30179T)（相似度94.8 %）是其系统发育最近的亲缘关系。Q-10是主要的呼吸醌。主要脂肪酸为特征8 （C18: 1ω6c和/或C18: 1ω7c）、特征3 （C16: 1ω6c和/或C16: 1ω7c）、C17: 1ω6c和C18: 1ω7c 11-甲基。极性脂质含有二磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰肌醇、鞘糖脂、四种未知的糖脂和一种未知的脂质。DNA G+C含量为65.1%。在本研究证据的基础上，菌株SSR2A-4-2T代表了Blastomonas属的一个新种，并以菌株SSR2A-4-2T （=CGMCC 1.15297T=DSM 103453T）为型菌株命名为Blastomonas marina sp. nov。
A Gram-stain-negative, facultatively anaerobic, dark-yellow-pigmented bacterium, named SSR2A-4-2T, was isolated from coastal water in the East China Sea. Cells were ovoid or short rods with peritrichous flagella and contained carotenoid in addition to bacteriochlorophyll a pigment. A phylogenetic dendrogram based on 16S rRNA gene sequences showed that strain SSR2A-4-2T formed a distinct clade with members of the genus Blastomonas, with Blastomonasnatatoria EY 4220T (=DSM 3183T) (similarity 95.6 %), Blastomonasursincola KR-99T (=DSM 9006T) (95.5 %) and Blastomonasaquatica PE4-5T (=JCM 30179T) (94.8 %) as its closest phylogenetic relatives. Q-10 was the predominant respiratory quinone. The major fatty acids were summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c), summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), C17 : 1ω6c and C18 : 1ω7c 11-methyl. The polar lipids contained diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, sphingoglycolipid, four unknown glycolipids and one unknown lipid. The DNA G+C content was 65.1 mol%. On the basis of the evidence presented in this study, strain SSR2A-4-2T represents a novel species of the genus Blastomonas, for which the name Blastomonas marina sp. nov. is proposed, with strain SSR2A-4-2T (=CGMCC 1.15297T=DSM 103453T) as the type strain.