Expression of iNOS mRNA and inhibitory effect of NO on uterine contractile activity in rats are determined by local rather than systemic factors of pregnancy.
Expression of iNOS mRNA and inhibitory effect of NO on uterine contractile activity in rats are determined by local rather than systemic factors of pregnancy.
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iNOS mRNA 的表达和 NO 对大鼠子宫收缩活动的抑制作用是由妊娠局部因素而不是全身因素决定的。
DOI：
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发表时间：
2004
影响因子：
3.5
通讯作者：
R. E. Gafield
中科院分区：
文献类型：
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作者：
T. Okawa;K. Asano;Hidenori Takahashi;S. Hashimoto;H. Anbe;A. Sato;R. E. Gafield
我们的目的是研究妊娠的局部或全身因素是否与诱导型一氧化氮合酶(INOS)mRNA的表达有关，并确定药物对提高大鼠子宫肌层cGMP水平的抑制作用。用RT-PCR方法检测未孕大鼠子宫组织及妊娠和未孕子宫角组织中inos基因的表达。此外，来自妊娠和非妊娠子宫角的子宫环被放置在Krebs-Henseleit溶液中，用于等长记录自发收缩。得到了二乙胺/一氧化氮复合体、8-溴-cGMP和选择性磷酸二酯酶V抑制剂的浓度-抑制关系。与未孕大鼠相比，子宫肌层中iNOS mRNA的表达在孕期增加，在第17天达到最大值，然后在第21天下降。妊娠第17天，妊娠子宫角中iNOS mRNA的表达高于未妊娠子宫角。最大限度地抑制磷酸二酯酶V和增加cGMP对非妊娠和妊娠子宫角自发收缩的抑制作用相似，而NO对前者的抑制作用较弱。结果提示，在诱导型一氧化氮合酶(INOS)mRNA表达最高时，NO向可溶性鸟苷环化酶信号转导需要局部妊娠因子。
Our purpose was to investigate whether the local or systemic factors of pregnancy are associated with inducible nitric oxide synthase (iNOS) mRNA expression and to determine the inhibitory effects of pharmacological agents that increase cGMP levels in rat myometrium. iNOS mRNA expression was determined in uterine tissues from nonpregnant rats and on day 17 of gestation in the pregnant and non-pregnant uterine horns by RT-PCR. In addition, uterine rings from the pregnant and non-pregnant uterine horns were placed in Krebs-Henseleit solution for isometric recordings of spontaneous contractions. Concentration-inhibition relationships to diethylamine/nitric oxide complex, 8-bromo-cGMP, and the selective phosphodiesterase V inhibitor were obtained. Compared to nonpregnant rats, expression of iNOS mRNA in myometrium increased during pregnancy, which was maximal on day 17, followed by a decrease on day 21 of gestation. Expression of iNOS mRNA at day 17 of gestation was greater in pregnant uterine horns than in nonpregnant ones. Maximal inhibition of phosphodiesterase V and increasing cGMP induced similar inhibition of spontaneous contractions in nonpregnant and pregnant uterine horns, while NO induced less inhibition in the former. The results suggest that the local pregnancy factor is needed for signal transduction from NO to soluble guanylate cyclase at a time when maximal expression of iNOS mRNA is evident.
怀孕会增加子宫肌层中的鸟苷 3,5-单磷酸，与一氧化氮的合成无关。
影响因子：
4.8
作者：
Weiner,CP;Knowles,RG;Nelson,SE;Stegink,LD
通讯作者：
Stegink,LD