CccS and CccP are involved in construction of cell surface components in the cyanobacterium Synechocystis sp. strain PCC 6803.
CccS and CccP are involved in construction of cell surface components in the cyanobacterium Synechocystis sp. strain PCC 6803.
复制标题
DOI：
10.1093/pcp/pcq081
复制
发表时间：
2010-07
影响因子：
4.9
通讯作者：
Hidehisa Yoshimura;Y. Kaneko;S. Ehira;Shizue Yoshihara;M. Ikeuchi;M. Ohmori
中科院分区：
文献类型：
--
作者：
Hidehisa Yoshimura;Y. Kaneko;S. Ehira;Shizue Yoshihara;M. Ikeuchi;M. Ohmori
我们以前已经确定了两个靶基因（slr1667和slr1668）的cAMP受体蛋白，SYCRP 1，在cAMP依赖性的方式进行转录调控。在这项研究中，我们调查了slr1667和slr1668（指定cccS和cccP，分别）的生物化学和免疫细胞化学的产品的本地化，并检查其破坏物的表型。CccS蛋白在培养基和酸溶性级分中检测到，所述酸溶性级分含有来自外膜外部的蛋白。cccS和cccP的突变体表现出或多或少相似的多效性表型。与野生型相比，两种干扰剂中分泌到培养基中或保留在外膜外侧的几种蛋白质大大减少。电子显微镜显示cccS破坏物缺乏负责运动性的厚皮利，并且cccP破坏物在其细胞表面上几乎没有可辨别的厚皮利。这两种干扰物在很大程度上分泌了比野生型多得多的黄色色素到培养基中。此外，破坏减少了可从胞外多糖层提取的UV吸收化合物的量。这些结果表明cccS和cccP基因参与了集胞藻菌株PCC 6803细胞表面组分的构建。
We have previously identified two target genes (slr1667 and slr1668) for transcriptional regulation by a cAMP receptor protein, SYCRP1, in a cAMP-dependent manner. For this study we investigated the localizations of products of slr1667 and slr1668 (designated cccS and cccP, respectively) biochemically and immunocytochemically, and examined the phenotypes of their disruptants. CccS protein was detected in the culture medium and the acid-soluble fraction containing proteins derived from outside the outer membrane. Disruptants of cccS and cccP showed a more or less similar pleiotropic phenotype. Several proteins secreted into the culture medium or retained on the outside of the outer membrane were greatly reduced in both disruptants compared with the wild type. Electron microscopy revealed that the cccS disruptant lacked the thick pili responsible for motility and that the cccP disruptant had almost no discernible thick pili on its cell surface. Both disruptants largely secreted far greater amounts of yellow pigments into the culture medium than did the wild type. Furthermore, the disruptions reduced the amount of UV-absorbing compound(s) extractable from the exopolysaccharide layer. These results suggest that the cccS and cccP genes are involved in the construction of cell surface components in Synechocystis sp. strain PCC 6803.