Phytochemical Analysis, Antibacterial Activity and Mode of Action of the Methanolic Extract of Scutellaria barbata Against Various Clinically Important Bacterial Pathogens
Phytochemical Analysis, Antibacterial Activity and Mode of Action of the Methanolic Extract of Scutellaria barbata Against Various Clinically Important Bacterial Pathogens
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DOI：
10.3923/ijp.2016.116.125
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发表时间：
2016-01-01
影响因子：
0.6
通讯作者：
Lu, Cong-Xiao
中科院分区：
文献类型：
--
作者：
Tang, Qi-Ling;Kang, Ai-Rong;Lu, Cong-Xiao
本研究的目的是评价半枝莲甲醇提取物的植物化学成分和抑菌作用。barbata）对一组临床上重要的细菌病原体的作用，沿着破译提取物的作用模式。采用液相色谱-质谱联用技术对活性提取物中的植物化学成分进行了分析。采用纸片扩散法和微量稀释法测定提取物的抑菌效果。使用扫描电子显微镜（SEM）评价浸提液引起的形态学变化。采用2 '，7'-二氯荧光素二乙酸酯（DCFDA）法检测5. 5的甲醇提取物诱导6株细菌产生活性氧（ROS）的情况。巴巴塔该提取物对所有供试细菌的生长均有抑制作用，且呈剂量依赖性。鼠伤寒是最敏感的病原体。在细菌细胞和提取物之间的40分钟接触时间内杀死的细菌细胞的百分比约为24.7%，在60分钟时间后增加到44.5%。该提取物还在所有细菌培养物中诱导有效的ROS产生以及细胞内蛋白质渗漏，其中在S.鼠伤寒。扫描电镜结果表明，该提取物引起了S.这表明ROS的产生可导致细菌细胞的膜损伤。本研究结果表明，S。半枝莲对多种细菌菌株具有潜在的抗菌作用，其抗菌作用是通过ROS产生、细胞内蛋白质渗漏和细菌细胞膜破裂介导的。
The objective of the current study was to evaluate the phytochemical composition and antibacterial effect of methanolic extract of Scutellaria barbata (S. barbata) against a panel of clinically important bacterial pathogens along with deciphering the mode of action of the extract. Liquid chromatography-mass spectrometry was performed to evaluate the phytochemical constituents of the bioactive extract. Disc diffusion assay and microdilution assay were used to test the antibacterial efficacy of the extract. Scanning Electron Microscopy (SEM) was used to evaluate the morphological changes induced by the extract. The 2', 7'-dichlorofluorescein diacetate (DCFDA) was used to detect the Reactive Oxygen Species (ROS) generation in these six bacterial strains induced by the methanol extract of 5. barbata. The extract inhibited cell growth of all tested bacteria dose-dependently with S. typhimurium being the most susceptible pathogen. The percentage of bacterial cells killed within a 40-min contact time between the bacterial cells and the extract was around 24.7% which increased to 44.5% after 60-min time. The extract also induced potent ROS generation as well is intracellular protein leakage in all bacterial cultures with maximum peak in S. typhimurium. The SEM results revealed that the extract induces deleterious morphological changes in the bacterial cell membrane of S. typhimurium indicating that ROS generation can lead to membrane damage in bacterial cells. The present findings indicate that S. barbata has potential antibacterial effect against a variety of bacterial strains and the antibacterial effect is mediated via ROS generation, intracellular protein leakage and rupture of bacterial cell membrane.