Central terminals of orofacial primary afferents and NADPH-diaphorase activity in the trigemino–solitary complex of rats
Central terminals of orofacial primary afferents and NADPH-diaphorase activity in the trigemino–solitary complex of rats
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DOI：
10.1016/s0006-8993(97)01210-9
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发表时间：
1998-01
期刊：
影响因子：
2.9
通讯作者：
M. Takemura;Atsutoshi Tsujio;K. Iwase;T. Shimada;Y. Shigenaga
中科院分区：
文献类型：
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作者：
M. Takemura;Atsutoshi Tsujio;K. Iwase;T. Shimada;Y. Shigenaga
烟酰胺腺嘌呤二核苷酸磷酸-黄递酶（NADPH-d）活性和中央终端领域的分支的下颌和鼓索神经可视化在同一时间使用跨神经节运输的麦胚凝集素结合辣根过氧化物酶组织化学。蓝NADPH-d阳性神经元在三叉神经脊束核口侧亚核背内侧区呈稀疏网络分布，在孤束核嘴侧外侧区呈密集网络分布。在尾侧亚核，标记神经元大多数位于浅区，少数位于大细胞区。口侧亚核和孤束核内NADPH-d阳性神经元与舌神经终末野重叠较多，部分与下齿槽神经和鼓索神经终末野重叠，与颏神经终末野重叠较少。三叉旁背内侧核和尾侧亚核内NADPH-d阳性神经元主要与舌神经终末野重叠，部分与下牙槽神经终末野和颏神经终末野重叠，与鼓索终末野不重叠。NADPH-d阳性神经元的数量在统计学上显着减少时，被认为是双边的口侧亚核和孤束核的舌神经被切断。舌神经或牙髓的炎症性损伤显着增加了NADPH-d阳性神经元的数量在口亚核，孤束核，和尾亚核。这些结果表明，在三叉神经和孤束核的NO/环GMP系统的差异调节跨突触三叉神经传入取决于核和感觉方式。
Nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) activity and the central terminal fields of branches of the mandibular and chorda tympani nerves were visualized histochemically at the same time using transganglionic transport of wheat germ agglutinin conjugated with horseradish peroxidase. The blue NADPH-d-positive neurons comprised a sparse network in the dorsomedial spinal trigeminal subnucleus oralis and a dense one in the rostral lateral division of the nucleus of the solitary tract. In the subnucleus caudalis, most labeled neurons were in the superficial zone, and smaller numbers were in the magnocellular zone. The NADPH-d-positive neurons in the subnucleus oralis and the nucleus of the solitary tract overlapped mostly with the transganglionically labeled terminal field from the lingual nerve, partly with the terminal field from the inferior alveolar and chorda tympani nerves, and rarely with the terminal field from the mental nerve. The NADPH-d-positive neurons in the dorsomedial paratrigeminal nucleus and subnucleus caudalis overlapped mostly with the terminal field from the lingual nerve, partly with the terminal field from the inferior alveolar and mental nerves and never with the terminal field from the chorda tympani. A statistically significant reduction in the number of NADPH-d-positive neurons was seen bilaterally in subnucleus oralis and the nucleus of the solitary tract when the lingual nerve was transected. Inflammatory insults to the lingual nerve or tooth pulps significantly increased the number of NADPH-d-positive neurons in subnucleus oralis, the nucleus of the solitary tract, and subnucleus caudalis. These results show that the NO/cyclic GMP system in the trigeminal and solitary nuclei is differentially regulated trans-synaptically by trigeminal afferents depending on the nucleus and sensory modality.