Galvanotactic control of collective cell migration in epithelial monolayers
Galvanotactic control of collective cell migration in epithelial monolayers
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DOI：
10.1038/nmat3891
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发表时间：
2014-04-01
期刊：
影响因子：
41.2
通讯作者：
Maharbiz, Michel M.
中科院分区：
文献类型：
--
作者：
Cohen, Daniel J.;Nelson, W. James;Maharbiz, Michel M.
许多正常和病理的生物学过程都涉及上皮细胞片的迁移。这是由于细胞信令网络和物理上耦合细胞的力之间的相互作用导致的复杂的紧急行为。在这里，我们证明了通过施加偏向于底层信号网络的电场，可以交互地引导上皮细胞的集体迁移。我们表明，复杂的时空线索是由上皮细胞局部解释的，导致快速、协调的反应，如集体掉头、分歧迁移和针对障碍的无节制迁移。我们观察到，外部控制的程度取决于细胞群体的大小和形状，以及细胞之间物理耦合的存在。总之，我们的结果为合理操纵组织的集体行为和材料属性提供了设计和工程原理。
Many normal and pathological biological processes involve the migration of epithelial cell sheets. This arises from complex emergent behaviour resulting from the interplay between cellular signalling networks and the forces that physically couple the cells. Here, we demonstrate that collective migration of an epithelium can be interactively guided by applying electric fields that bias the underlying signalling networks. We show that complex, spatiotemporal cues are locally interpreted by the epithelium, resulting in rapid, coordinated responses such as a collective U-turn, divergent migration, and unchecked migration against an obstacle. We observed that the degree of external control depends on the size and shape of the cell population, and on the existence of physical coupling between cells. Together, our results offer design and engineering principles for the rational manipulation of the collective behaviour and material properties of a tissue.