Multiple Phenotypic Changes Associated with Large-Scale Horizontal Gene Transfer
Multiple Phenotypic Changes Associated with Large-Scale Horizontal Gene Transfer
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DOI：
10.1371/journal.pone.0102170
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发表时间：
2014-07-21
期刊：
影响因子：
3.7
通讯作者：
Baltrus, David A.
中科院分区：
文献类型：
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作者：
Dougherty, Kevin;Smith, Brian A.;Baltrus, David A.
水平基因转移通常会导致受体生物体内的表型变化，而不依赖于任何直接的进化益处。而水平转移的次级表型效应（即，生长速率的变化）已经在使用相对小的质粒和噬菌体的各种系统中得到证实和研究，但是对于转移较大区域后这种代价的大小或数目知之甚少。在这里，我们描述了许多表型的变化，发生后，大规模的水平转移事件（类似于1 Mb megaplasma）内施氏假单胞菌，包括敏化各种应力以及细菌行为的变化。这些结果突出了水平转移在细菌基因组内改变多效性关系和细胞网络的力量。它们还提供了一个重要的背景，说明转移的次级效应如何使物种之间的进化轨迹和相互作用产生偏差。最后，这些结果和系统提供了一个基础，以调查进化的后果，在真实的时间作为新获得的区域被改善和整合到新的基因组环境。
Horizontal gene transfer often leads to phenotypic changes within recipient organisms independent of any immediate evolutionary benefits. While secondary phenotypic effects of horizontal transfer (i.e., changes in growth rates) have been demonstrated and studied across a variety of systems using relatively small plasmids and phage, little is known about the magnitude or number of such costs after the transfer of larger regions. Here we describe numerous phenotypic changes that occur after a large-scale horizontal transfer event (similar to 1 Mb megaplasmid) within Pseudomonas stutzeri including sensitization to various stresses as well as changes in bacterial behavior. These results highlight the power of horizontal transfer to shift pleiotropic relationships and cellular networks within bacterial genomes. They also provide an important context for how secondary effects of transfer can bias evolutionary trajectories and interactions between species. Lastly, these results and system provide a foundation to investigate evolutionary consequences in real time as newly acquired regions are ameliorated and integrated into new genomic contexts.