Immunohistochemical localization of large chondroitin sulphate proteoglycan in porcine gingival epithelia
Immunohistochemical localization of large chondroitin sulphate proteoglycan in porcine gingival epithelia
复制标题
DOI：
10.1076/ejom.39.2.99.7372
复制
发表时间：
2001-04-01
期刊：
影响因子：
--
通讯作者：
Kaku, T
中科院分区：
文献类型：
--
作者：
Abiko, Y;Nishimura, M;Kaku, T
在这项研究中，我们报告的多功能蛋白聚糖在健康猪牙龈上皮的免疫组织化学定位。单克隆抗体（mAb）5D5特异性识别大硫酸软骨素蛋白聚糖的核心蛋白，如多能蛋白聚糖、神经聚糖和短蛋白聚糖，但不识别聚集蛋白聚糖的核心蛋白。由于神经聚糖和短蛋白聚糖似乎对神经组织具有特异性，因此本研究中检查的大硫酸软骨素蛋白聚糖最有可能是多功能蛋白聚糖。在附着的牙龈上皮的角化层中，基底细胞和棘细胞表面显示出对mAb 5D5的强烈染色。在沟上皮的角化不全层中，定位仅限于基底层和下棘层。在结合上皮中，在釉质表面附近的一个或两个细胞层中观察到强染色。免疫电镜显示高密度沉积的5D5免疫反应上皮细胞表面。在釉质表面，5D5免疫反应定位于牙角质层的交界上皮，但不存在于内部基底层。这些结果表明，多功能蛋白聚糖，一个大的硫酸软骨素蛋白聚糖，参与上皮分化和向下生长。
In this study, we report the immunohistochemical localization of versican in healthy porcine gingival epithelia. The monoclonal antibody (mAb), 5D5, specifically recognizes core proteins of large chondroitin sulphate proteoglycans such as versican, neurocan and brevican, but not the core protein of aggrecan. Because neurocan and brevican appear to be specific to nervous tissue, the large chondroitin sulphate proteoglycans examined in this study is most likely versican. In the keratinized layer of the attached gingival epithelium, the basal and spinous cell surfaces showed intense staining for mAb 5D5. In the parakeratinized layer of the sulcus epithelium, the localization was restricted to the basal and lower spinous layers. In the junctional epithelium, intense staining was observed in one or two cell layers near the enamel surface. Immunoelectron microscopy revealed high-density depositions of 5D5 immunoreactivity on epithelial cell surfaces. At the enamel surface, 5D5 immunoreactivity was localized to the dental cuticle of the junctional epithelium but was not present in the internal basal lamina. These results suggest that versican, a large chondroitin sulphate proteoglycan, is involved in epithelial differentiation and downgrowth.