Sponge-induced angiogenesis in mice and the pharmacological reactivity of the neovasculature quantitated by a fluorimetric method
Sponge-induced angiogenesis in mice and the pharmacological reactivity of the neovasculature quantitated by a fluorimetric method
复制标题
DOI：
10.1006/mvre.1997.2047
复制
发表时间：
1997-11-01
影响因子：
3.1
通讯作者：
Beraldo, WT
中科院分区：
文献类型：
--
作者：
Andrade, SP;Machado, RDP;Beraldo, WT
海绵诱导的小鼠血管生成和药理学反应的新血管已被确定的荧光法。皮下、皮内和植入物内给予荧光素钠后的药代动力学研究产生了一个双相曲线，根据该曲线可以估计染料吸收和消除的t（1/2）。在植入后第1、4、7、10和14天局部注射染料后，体循环中荧光染料产生的发射的测量反映了植入物内和周围血流的发展以及血管生成部位与体循环的相互作用。血流中荧光峰的tm值从初始值6.41 +/- 0.28 min（无血管植入物）稳定降低至完全血管化植入物中的2.78 +/- 0.23 min（第14天）。新生血管对ET-1的反应性呈剂量依赖性，与皮肤血管相似。相比之下，在植入物血管中未检测到对组胺的反应性，而皮肤中存在组胺。这些结果表明，新生血管的药理学反应不同于成熟血管的反应。血管生成的刺激（bFGF，300纳克，每天）减少锡的荧光峰，而地塞米松（1毫克/公斤）增加it. Parallel组织学研究证实了功能的结果。这些观察结果表明，该试验适用于研究血管生成、新生血管的功能和药理学表征以及血管生成部位与体循环的相互作用。(C)1997年学术出版社。
Sponge-induced angiogenesis in mice and pharmacological reactivity of the neovasculature have been determined by a fluorimetric method. Pharmacokinetic studies following subcutaneous, intradermal, and intraimplant administration of sodium fluorescein resulted in a biphasic curve from which estimation of t(1/2) for absorption and elimination of the dye were possible. Following topical injection of the dye at days 1, 4, 7, 10, and 14 postimplantation, measurement of fluorchrome generated emission in the systemic circulation reflected the development of blood flow in and around the implants and the interaction of the angiogenic site with the systemic circulation. The tm values for the fluorescence peak in the bloodstream decreased steadily from an initial value of 6.41 +/- 0.28 min (avascular implant) to 2.78 +/- 0.23 min in fully vascularized implants (day 14). The reactivity of the neovasculature to ET-1 was dose-dependent and similar to the skin vasculature. By contrast, no reactivity to histamine was detected in the implant blood vessels, whereas it was present in the skin. These results show that the pharmacological response of the neovasculature differs from the response of mature blood vessels. The angiogenic stimulus (bFGF, 300 ng daily) decreased tin for the fluorescence peak, whereas dexamethasone (1 mg/kg) increased it. Parallel histological studies corroborated the functional findings. These observations indicate the suitability of this assay to study angiogenesis, functional and pharmacological characterization of the neovasculature, and the interaction of the angiogenic site with the systemic circulation. (C) 1997 Academic Press.