Antimalarial activity of extracts and alkaloids isolated from six plants used in traditional medicine in Mali and Sao Tome
Antimalarial activity of extracts and alkaloids isolated from six plants used in traditional medicine in Mali and Sao Tome
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DOI：
10.1002/ptr.1025
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发表时间：
2002-11-01
影响因子：
7.2
通讯作者：
Balansard, G
中科院分区：
文献类型：
--
作者：
Ancolio, C;Azas, N;Balansard, G
从采自马里的四种植物的不同部位制备甲醇和氯仿提取物：Guiera senegalensis（Gmel.）使君子科（Combretaceae）、远花铁菊（Feretia apodanthera（Del.）茜草科（Rubiaceae）、细叶风毛草（Combretum micranthum（Don.）使君子科（Combretaceae）、长梗一叶藤Securidaca longepedunculata（Fres.）本文报道了远志科（Polygalaceae）和采自圣多美（Sao托姆）的两种植物：安哥拉Pycnanthus angolensis（Welw.）肉豆蔻科（Myristicaceae）和海巴戟天（Morinda citrifolia（Benth.）通过流式细胞术评估茜草科植物的体外抗疟活性和对人单核细胞（THP 1细胞）的细胞毒性作用。无柄菊叶的甲醇提取物和塞内加尔桂根的氯仿提取物具有明显的抗疟活性。从圭亚那的活性提取物中分离的两种生物碱，barman和tetrahydroharman，显示出抗疟活性（IC 50低于4 μ/mL），并显示出对THP 1的低毒性。此外，在细胞周期的S期的THP 1细胞的减少，用哈曼和四氢哈曼处理后，可能是由于总蛋白质合成的抑制。版权所有（C）2002约翰威利父子有限公司
Methanol and chloroform extracts were prepared from various parts of four plants collected in Mali: Guiera senegalensis (Gmel.) Combretaceae, Feretia apodanthera (Del.) Rubiaceae, Combretum micranthum (Don.) Combretaceae, Securidaca longepedunculata (Fres.) Polygalaceae and two plants collected in Sao Tome: Pycnanthus angolensis (Welw.) Myristicaceae and Morinda citrifolia (Benth.) Rubiaceae were assessed for their in vitro antimalarial activity and their cytotoxic effects on human monocytes (THP1 cells) by flow cytometry. The methanol extract of leaves of Feretia apodanthera and the chloroform extract of roots of Guiera senegalensis exhibited a pronounced antimalarial activity. Two alkaloids isolated from the active extract of Guiera senegalensis, barman and tetrahydroharman, showed antimalarial activity (IC50 lower than 4 mu/mL) and displayed low toxicity against THP1. Moreover, the decrease of THP1 cells in S phase of the cell cycle, after treatment with harman and tetrahydroharman, was probably due to an inhibition of total protein synthesis. Copyright (C) 2002 John Wiley Sons, Ltd.