MicroCT analysis of hydroxyapatite bone repair scaffolds created via three-dimensional printing for evaluating the effects of scaffold architecture on bone ingrowth
MicroCT analysis of hydroxyapatite bone repair scaffolds created via three-dimensional printing for evaluating the effects of scaffold architecture on bone ingrowth
复制标题
DOI：
10.1002/jbm.a.31484
复制
发表时间：
2008-05-01
影响因子：
4.9
通讯作者：
Parsons, J. Russell
中科院分区：
文献类型：
--
作者：
Simon, Joshua L.;Rekow, E. Dianne;Parsons, J. Russell
最近的研究表明，现在可以使用3d打印技术构建具有紧密孔径分布和控制几何形状的组织工程骨修复支架（QDP）。本研究采用2 × 2因子设计评估了8周和16周兔环钻缺损的两个羟基磷灰石（HA）直径8毫米的可控结构椎间盘。输入参数分别为时间和支架空隙体积。从MicroCT数据中提取三个输出变量：相对于总感兴趣区域的骨体积长入，相对于可用长入体积的骨体积长入和软组织体积。实验测量了两组——第1组：500 μ m × 500 μ m的通道，与支架长轴平行，从底部向上穿透3mm。第2组：800 μ m × 800 μ m支柱，间隔500 pm，在每个印刷层中垂直放置。三维MicroCT扫描和未钙化的组织切片显示植入物与周围组织融合良好，大量骨向内生长。因子分析表明，时间是软组织长入的最大决定因素，而时间及其与空隙体积的相互作用是骨量长入的最大决定因素。(C) 2007 Wiley期刊公司
Recent studies have shown that it is now possible to construct tissue-engineered bone repair scaffolds with tight pore size distributions and controlled geometries using 3-D Printing techniques QDP). This study evaluated two hydroxyapatite (HA) 8-mm diameter discs with controlled architectures in a rabbit trephine defect at 8 and 16 weeks using a 2 X 2 factorial design. Input parameters were time and scaffold void volume at two levels. Three output variables were extracted from MicroCT data: bone volume ingrowth with respect to total region of interest, bone volume ingrowth with respect to available ingrowth volume, and soft tissue volume. The experiment measured two groups-Group 1: 500-mu m X 500-mu m channels parallel to the scaffold's long axis and penetrating up 3-mm from the bottom. Group 2: 800-mu m X 800-mu m struts spaced 500 pm apart set perpendicularly to each other in each printed layer. Rendered 3-dimensional MicroCT scans and undecalcitied histological slides of implants revealed good integration with the surrounding tissue, and a sizeable amount of bone ingrowth into the device. Factorial analysis revealed that the effects of time were the greatest determinant of soft tissue ingrowth, while time and its interaction with void volume were the greatest determinants of bone volume ingrowth with respect to both total and available volume. (C) 2007 Wiley Periodicals, Inc.