A GENETIC MAP OF POLIOVIRUS TEMPERATURE-SENSITIVE MUTANTS
A GENETIC MAP OF POLIOVIRUS TEMPERATURE-SENSITIVE MUTANTS
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DOI：
10.1016/0042-6822(68)90287-0
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发表时间：
1968-01-01
期刊：
影响因子：
3.7
通讯作者：
COOPER, PD
中科院分区：
文献类型：
--
作者：
COOPER, PD
规范了脊髓灰质炎病毒ts突变体间重组的条件和ts+重组体的检测。每对突变体的平均重组频率均具有重复性和特征性，为总背景还原率的5.2 ~ 31.4倍，范围为0.02% ~ 0.85%。对胍抗性（ts- 28g）突变体的3因子杂交证实了某些基因序列，表明重组频率是可加性的。得到了包含一个连锁群的加性线性遗传图谱。ts- 28g的g特征似乎是在一个可以精确定位在地图中间的单一位点突变的结果。重组随时间的变化是非随机的，大多数交配事件发生在生长周期的早期。
The conditions affecting recombination between ts mutants of poliovirus and the assay of ts+ recombinants were standardized. The mean recombinant frequencies were then reproducible and characteristic of each pair of mutants, being between 5.2 and 31.4 times the total background reversion rates and ranging from 0.02% to 0.85%. Certain genetic sequences were proved by 3-factor crosses involving a mutant adapted to guanidine resistance (ts-28 g), and these showed that recombination frequencies were additive. An additive linear genetic map comprising one linkage group was obtained. The g character of ts-28 g seemed to result from mutation at a single site that could be accurately located near the middle of the map. Recombination was nonrandom with time, most of the mating events occurring early in the growth cycle.