POSTNATAL CHANGES IN EXPRESSION OF VESICULAR GLUTAMATE TRANSPORTERS IN THE MAIN OLFACTORY BULB OF THE RAT
POSTNATAL CHANGES IN EXPRESSION OF VESICULAR GLUTAMATE TRANSPORTERS IN THE MAIN OLFACTORY BULB OF THE RAT
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DOI：
10.1016/j.neuroscience.2009.02.048
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发表时间：
2009-05-05
期刊：
影响因子：
3.3
通讯作者：
Hisano, S.
中科院分区：
文献类型：
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作者：
Ohmomo, H.;Ina, A.;Hisano, S.
嗅觉信息最初是通过嗅球中GABA能投射神经元和GABA能中间神经元之间复杂的突触相互作用来处理的。尽管有报道称延髓神经元和网络甚至在出生后也会发育，但关于延髓能神经元的发育仍有很多未知之处。为了解决这个问题，我们研究了泡状谷氨酸转运体（VGLUT 1和VGLUT 2）在大鼠的主要嗅球出生后的个体发育，使用原位杂交，免疫组织化学，以及它们的组合。原位杂交数据表明，VGLUT1 mRNA强烈表达在分化的二尖瓣细胞和较小的细胞的二尖瓣细胞层（MCL）在出生后第1天（P1），也在较低的水平在小型和中型细胞，推测簇状细胞群，从P5向前的外部丛状层（EPL）。VGLUT2 mRNA表达在许多MCL细胞群体的P1，也在小和中等大小的细胞的EPL在几乎相同的水平MCL细胞之间的P5和P7，并成为明显较弱的强度在MCL比在EPL从P10起。与VGLUT1 mRNA不同，在肾小球间区的小细胞中也发现了表达。与这些数据部分一致，免疫组化分析表明，二尖瓣和EPL细胞的亚群VGLUT 1或VGLUT 2染色，与前细胞共表达两种亚型，直到P5。此外，结合荧光原位杂交-免疫组织化学双标记的P10球显示，无论是VGLUT 1还是VGLUT 2 mRNA的表达在GABA能或多巴胺能肾小球周围细胞，这意味着他们的表达在其他肾小球周围细胞亚类，外部簇状细胞和/或短轴突细胞。因此，本研究表明，在出生后发育的早期不同的大鼠延髓神经元时空表达的两个VGLUT亚型作为一个特定的囊泡运输系统，特别是谷氨酸介导的神经生物学事件。(C)2009年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Olfactory information is initially processed through intricate synaptic interactions between glutamatergic projection neurons and GABAergic interneurons in the olfactory bulb. Although bulbar neurons and networks have been reported to develop even postnatally, much is yet unknown about the glutamatergic neuron development. To address this issue, we studied the postnatal ontogeny of vesicular glutamate transporters (VGLUT1 and VGLUT2) in the main olfactory bulb of rats, using in situ hybridization, immunohistochemistry, and their combination. In situ hybridization data showed that VGLUT1 mRNA is intensely expressed in differentiating mitral cells and smaller cells of the mitral cell layer (MCL) on postnatal day 1 (P1), and also at lower levels in small- and medium-sized cells, presumably tufted cell populations, of the external plexiform layer (EPL) from P5 onward. VGLUT2 mRNA was expressed in many MCL cell populations on P1, also in small- and medium-sized cells of the EPL at almost the same level as MCL cells between P5 and P7, and became apparently less intense in the MCL than in the EPL from P10 onward. The expression, unlike VGLUT1 mRNA, was also found in small-sized cells of the interglomerular region. In partial agreement with these data, immunohistochemical analyses demonstrated that subsets of mitral and EPL cells are stained for VGLUT1 or VGLUT2, with the former cells coexpressing both subtypes until P5. Moreover, a combined fluorescence in situ hybridization-immunohistochemical dual labeling of the P10 bulb revealed that neither VGLUT1 nor VGLUT2 mRNA is expressed in GABAergic or dopaminergic periglomerular cells, implying their expression in other periglomerular cell subclasses, external tufted cells and/or short-axon cells. Thus, the present study suggests that early in the postnatal development distinct glutamatergic bulbar neurons of rats express spatiotemporally either or both of the two VGLUT subtypes as a specific vesicular transport system, specifically contributing to glutamate-mediated neurobiological events. (C) 2009 IBRO. Published by Elsevier Ltd. All rights reserved.