Vesicle association and exocytosis at ribbon and extraribbon sites in retinal bipolar cell presynaptic terminals.
Vesicle association and exocytosis at ribbon and extraribbon sites in retinal bipolar cell presynaptic terminals.
复制标题
视网膜双极细胞突触前末梢丝带和丝带外位点的囊泡关联和胞吐作用。
DOI：
10.1073/pnas.0709067105
复制
发表时间：
2008
影响因子：
11.1
通讯作者：
Zenisek,David
中科院分区：
文献类型：
--
作者：
Zenisek,David
突触囊泡通过囊泡对接和胞吐作用释放神经递质。虽然这些步骤已经很好地建立，但很难直接在活突触中观察和测量这些速率。在这里，通过结合直接成像的单个突触囊泡和突触带，我测量的属性的囊泡对接和诱发和自发释放的丝带和extraribrillary位置在一个带状突触终端，金鱼视网膜双极细胞。在没有刺激的情况下，被捕获的囊泡附近的丝带协会紧密，只有很少解开或进行自发胞吐。相比之下，在异常位点的囊泡捕获不太稳定，并且自发胞吐以更高的速率发生。响应于刺激，胞吐事件集群附近的丝带，但没有证据表明集群远离丝带网站。总之，这里的结果表明，虽然囊泡可以关联和融合附近和远离突触部位，囊泡在突触带关联更稳定，融合更紧密地联系在一起的刺激。
Synaptic vesicles release neurotransmitter by following a process of vesicle docking and exocytosis. Although these steps are well established, it has been difficult to observe and measure these rates directly in living synapses. Here, by combining the direct imaging of single synaptic vesicles and synaptic ribbons, I measure the properties of vesicle docking and evoked and spontaneous release from ribbon and extraribbon locations in a ribbon-type synaptic terminal, the goldfish retinal bipolar cell. In the absence of a stimulus, captured vesicles near ribbons associate tightly and only rarely undock or undergo spontaneous exocytosis. By contrast, vesicle capture at outlier sites is less stable and spontaneous exocytosis occurs at a higher rate. In response to a stimulus, exocytic events cluster near ribbons, but show no evidence of clustering away from ribbon sites. Together, the results here indicate that, although vesicles can associate and fuse both near and away from synaptic sites, vesicles at synaptic ribbons associate more stably and fusion is more tightly linked to stimuli.