Cloning and characterization of an ATPase gene from Pneumocystis carinii which closely resembles fungal H+ ATPases.
Cloning and characterization of an ATPase gene from Pneumocystis carinii which closely resembles fungal H+ ATPases.
复制标题
卡氏肺孢子虫 ATP 酶基因的克隆和表征，该基因与真菌 H ATP 酶非常相似。
DOI：
10.1111/j.1550-7408.1995.tb01584.x
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Stringer,JR
中科院分区：
文献类型：
--
作者：
Meade,JC;Stringer,JR
A gene encoding a P‐type cation translocating ATPase was cloned from a genomic library of rat‐derivedPneumocystis carinii.The nucleotide sequence of the gene contains a 2781 base‐pair open reading frame that is predicted to encode a 101, 401 dalton protein composed of 927 amino acids. TheP. cariniiATPase protein (pcal) is 69–75% identical when compared with eight proton pumps from six fungal species. ThePneumocystisATPase is less than 34% identical to ATPase proteins from protozoans, vertebrates or the Ca++ATPases of yeast. TheP. cariniiATPase contains 115 of 121 residues previously identified as characteristic of H+ATPases. Alignment of thePneumocystisand fungal proton pumps reveals five homologous domains specific for fungal H+ATPases.
