Two novel proteins recruited by synaptonemal complex protein 1 (SYCP1) are at the centre of meiosis
Two novel proteins recruited by synaptonemal complex protein 1 (SYCP1) are at the centre of meiosis
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DOI：
10.1242/jcs.02402
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发表时间：
2005-06-15
影响因子：
4
通讯作者：
Cooke, HJ
中科院分区：
文献类型：
--
作者：
Costa, Y;Speed, R;Cooke, HJ
哺乳动物减数分裂的完成依赖于联会复合体的形成，联会复合体是一种在前期I将同源染色体物理连接起来的三部分结构。联会复合体的几个组成部分是已知的，包括成分的凝聚核心，轴/侧元素和横向丝。此前尚未发现任何蛋白质是中心元件的唯一组成部分。一些联会复合体蛋白的突变导致减数分裂受损。在人类中，男性不育的病例与未能建立联会复合体有关。为了寻找减数分裂机制的新组成部分，我们使用了微阵列表达谱的数据，发现两种蛋白质仅定位于哺乳动物联会复合体的中心元件。这些新的蛋白质，SYCE 1和CESC 1，与横丝蛋白SYCP 1相互作用，其定位到中央元件似乎取决于SYCP 1的招聘。这表明SYCE 1和CESC 1在联会复合体组装中的作用，可能也是稳定性和重组。
Completion of meiosis in mammals depends on the formation of the synaptonemal complex, a tripartite structure that physically links homologous chromosomes during prophase I. Several components of the synaptonemal complex are known, including constituents of the cohesin core, the axial/lateral element and the transverse filaments. No protein has previously been identified as an exclusive component of the central element. Mutations in some synaptonemal-complex proteins results in impaired meiosis. In humans, cases of male infertility have been associated with failure to build the synaptonemal complex. To search for new components of the meiotic machinery, we have used data from microarray expression profiling and found two proteins localising solely to the central element of the mammalian synaptonemal complex. These new proteins, SYCE1 and CESC1, interact with the transverse filament protein SYCP1, and their localisation to the central element appears to depend on recruitment by SYCP1. This suggests a role for SYCE1 and CESC1 in synaptonemal-complex assembly, and perhaps also stability and recombination.