Chemiluminescence determination of tetracycline based on radical production in a basic acetonitrile–hydrogen peroxide reaction
Chemiluminescence determination of tetracycline based on radical production in a basic acetonitrile–hydrogen peroxide reaction
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DOI：
10.1016/j.aca.2003.10.035
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发表时间：
2004-02
影响因子：
6.2
通讯作者：
C. Lau;Jianzhong Lu;M. Kai
中科院分区：
文献类型：
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作者：
C. Lau;Jianzhong Lu;M. Kai
本文报道了一种测定四环素(TC)的化学发光法。这是基于观察到在碱性溶液中，四环素能极大地增强H_2O_2和乙腈之间的超弱化学发光。光发射强烈且寿命长，即使是简单的设置，也可以实现高灵敏度。线性范围为5μM-200 nm，检出限为60 nm。推测这一新反应涉及单线态氧和羟基自由基，化学发光物质属于氧化的四环素，而不是四环素本身。作为初步应用，该方法已成功地应用于药物中四环素的测定。
Here we report a rather simple and convenient chemiluminescence (CL) method for the determination of tetracycline (TC). It is based on the observation that tetracycline can greatly enhance an ultra-weak CL between H2O2and acetonitrile in a basic alkaline solution. Light emission is intense and long-lived, and even with a simple set-up, a high sensitivity could be achieved. The linear range was 5μM–200nM with a detection limit of 60nM. Singlet oxygen and hydroxyl radical are suggested to be involved in this new reaction, and the CL emitter is classified as the oxidized tetracycline, not tetracycline itself. As a preliminary application, this simple method has been successfully applied into the determination of tetracycline in the drugs.