Psychosocial modulation of cytokine-induced natural killer cell activity in older adults
Psychosocial modulation of cytokine-induced natural killer cell activity in older adults
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DOI：
10.1097/00006842-199605000-00010
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发表时间：
1996-05-01
影响因子：
3.3
通讯作者：
Glaser, R
中科院分区：
文献类型：
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作者：
Esterling, BA;KiecoltGlaser, JK;Glaser, R
本研究的目的是解决之前观察到的与慢性应激相关的自然杀伤（NK）细胞毒性变化的细胞和心理机制。我们比较了28名阿尔茨海默病（AD）患者的现任和前任配偶照顾者和29名对照受试者。NK细胞富集（E-NK）使用4步程序，导致细胞制备包括88.2%的NK细胞。然后将这些细胞与重组干扰素- γ (rifn - γ)或重组白细胞介素-2 （rIL-2）孵育65小时。虽然从阿尔兹海默病患者死亡至今，前护理人员的平均时间已超过3年，但目前和以前的护理人员在E-NK细胞对rifn - γ和rIL-2的反应上没有差异。然而，与对照组相比，联合护理组的E-NK细胞反应明显受到抑制，这与我们实验室之前的报告一致。较高的E-NK细胞对每种细胞因子的反应与积极情绪和有形社会支持水平的提高有关，与抑郁水平无关。初步数据表明，照顾者压力导致的NK细胞功能缺陷对rifn - γ和rIL-2的反应可能与非NK细胞无关。最后，我们的数据与其他关于社会支持在免疫调节中的作用的研究一致。
The objective of this study was to address the cellular and psychological mechanisms underlying previously observed changes in natural killer (NK) cell cytotoxicity associated with chronic stress. We compared 28 current and former spousal caregivers of patients with Alzheimer's disease (AD) and 29 control subjects. NK cells were enriched (E-NK) using a 4-step procedure that resulted in a cell preparation consisting of 88.2% NK cells. These cells were then incubated with either recombinant interferon-gamma (rIFN-gamma) or recombinant interleukin-2 (rIL-2] for 65 hours. Although an average of over 3 years had elapsed since the death of the patient with AD for the former caregivers, current and former caregivers did not differ in the E-NK cell responses to rIFN-gamma and rIL-2. However, the E-NK cell response for the combined caregiver group was significantly suppressed compared with controls, which is consistent with a previous report from our laboratory. Higher E-NK cell responses to each cytokine were associated with heightened levels of positive emotional and tangible social support, independent of levels of depression. Preliminary data suggest that defects of NK cell function in response to rIFN-gamma and rIL-2 as a consequence of caregiver stress may be independent of non-NK cells. Finally, our data are consistent with other studies regarding the role of social support in immune modulation.