Effect of quinoid redox mediators during azo dye decolorization by anaerobic sludge: Considering the catalyzing mechanism and the methane production
Effect of quinoid redox mediators during azo dye decolorization by anaerobic sludge: Considering the catalyzing mechanism and the methane production
复制标题
醌型氧化还原介体在厌氧污泥偶氮染料脱色过程中的作用：考虑催化机制和甲烷产生
DOI：
10.1016/j.ecoenv.2020.110859
复制
发表时间：
2020-10-01
影响因子：
6.8
通讯作者：
Zhao, Min
中科院分区：
文献类型：
--
作者：
Cui, Daizong;Zhang, Miao;Zhao, Min
The effects of quinoid compounds on azo dyes decolorization were studied. Compared with other quinones, menadione was the most effective at aiding azo dye decolorization. Sodium formate was a suitable carbon source for the anaerobic decolorization system. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis indicated that the microbial structure changed in response to varying carbon sources. Phylogenetic analysis showed that the anaerobic sludge was consisted mainly of nine genera. The mechanism studies showed that the biotransformation of menadione to its hydroquinone form was the rate-limiting step in the dye decolorization process. Moreover, study of the electron transfer mechanism of quinone-mediated reduction showed that azo dye decolorization is not a specific reaction. The NADH chain was involved in the decolorization process. The methane production test indicated that azo dyes had an inhibitory effect on methane production. However, supplementation with a redox mediator could recover the inhibited methanogenesis. High-throughput sequencing analysis revealed that the methanogenic archaeal community was altered in the anaerobic sludge with or without azo dyes and the redox mediator.
