Isolation of digested sludge-assimilating fungal strains and their potential applications.
Isolation of digested sludge-assimilating fungal strains and their potential applications.
复制标题
消化污泥同化真菌菌株的分离及其潜在应用。
DOI：
10.1111/jam.12266
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发表时间：
2013
影响因子：
4
通讯作者：
A.
中科院分区：
文献类型：
--
作者：
Fujii;K.;Kai;Y.;Matsunobu;S.;Sato;H.;Mikami;A.
消化污泥是城市污泥厌氧消化的主要产物，具有很强的生物降解性。在这项研究中，我们分离和表征DS-同化真菌从soil.Methods和ResultsWe试图分离DS-同化菌株的富集培养使用DS作为营养源，但微生物的生长没有观察到在任何文化。为了消除DS中金属对微生物生长的抑制作用，随后使用酸处理的DS进行富集，并从传代培养物中分离出8种真菌菌株。培养1周后，观察到污泥至少减少10-30%，延长培养时间可进一步减少污泥。所有菌株均产木聚糖酶、几丁质酶和角蛋白酶。系统发育分析表明，分离菌株为青霉属（Penicillium）、镰刀菌属（Fusarium）、毛壳菌属（Chaetomium）、小克银汉霉属（Cunninghamella）、奈氏菌属（Neutralya）和丛枝菌属（Umbelopsis）。结论本研究首次报道了DS-同化菌株的分离，为利用DS作为底物生产微生物酶提供了依据。由于污泥-菌丝复合体中的木聚糖、几丁质和角蛋白被认为是部分解聚的，因此这种材料也可以用作容易获得的肥料。
AimsDigested sludge (DS) is a major waste product of anaerobic digestion of sewage sludge and is resistant to biodegradation. In this study, we isolated and characterized DS‐assimilating fungi from soil.Methods and ResultsWe tried to isolate DS‐assimilating strains by enrichment culture using DS as the nutrient source, but microbial growth was not observed in any culture. To eliminate the inhibitory effect of metals in DS on microbial growth, acid‐treated DS was subsequently used for enrichment, and eight fungal strains were isolated from the subcultures. At least 10–30% reduction in sludge was observed after 1‐week cultivation, and prolonged cultivation led to further sludge reduction. All isolates produced xylanase, chitinase and keratinase. Phylogenetic analysis revealed that the isolates werePenicillium,Fusarium,Chaetomium,Cunninghamella,NeosartoryaandUmbelopsis. Some isolates were suggested novel species.ConclusionsTo the best of our knowledge, our study is the first to report the isolation of DS‐assimilating strains.Significance and Impact of the StudyThese isolates may be useful for commercial production of microbial enzymes using DS as the substrate. Because xylan, chitin and keratin in sludge–hyphae complexes are considered to be partially depolymerized, this material could also be utilized as a readily available fertilizer.