Direct ionization of biological tissue for mass spectrometric analysis
Direct ionization of biological tissue for mass spectrometric analysis
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DOI：
10.1039/c2an16223g
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发表时间：
2012-01-01
期刊：
影响因子：
4.2
通讯作者：
Yao, Zhong-Ping
中科院分区：
文献类型：
--
作者：
Hu, Bin;Lai, Ying-Han;Yao, Zhong-Ping
分子水平上的生物组织分析在生物学、医学和临床研究中具有重要意义。在这份手稿中，我们报告植物和动物组织都可以在环境条件下直接电离并通过质谱分析。通过添加一些溶剂并施加高电压，可以在生物组织的尖端诱导喷雾电离并观察质谱。已经对各种植物和动物组织进行了测试，并且可以在光谱中检测到脂质、生物碱、糖苷、木脂素、药物和蛋白质等化合物。这项新技术为组织分析提供了一种简单而快速的方法，并允许观察其他电离技术无法检测到的化合物。
Analysis of biological tissue at a molecular level is of great importance in biological, medical and clinical studies. In this manuscript, we report that both plant and animal tissues can be directly ionized and analyzed by mass spectrometry under ambient conditions. By adding some solvents and applying a high voltage, spray ionization can be induced at the tip of biological tissue and a mass spectrum can be observed. Various plant and animal tissues have been tested and compounds such as lipids, alkaloids, glucosides, lignans, pharmaceuticals and proteins could be detected in the spectra. This new technique provides a simple and rapid method for tissue analysis and allows observation of compounds that cannot be detected by other ionization techniques.