Digitally encoded silica microparticles for multiplexed nucleic acid detection
Digitally encoded silica microparticles for multiplexed nucleic acid detection
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用于多重核酸检测的数字编码二氧化硅微粒
DOI：
10.1039/c7cc01974b
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发表时间：
2017
影响因子：
4.9
通讯作者：
Li Jiong
中科院分区：
文献类型：
--
作者：
Xu Weiwei;Chen Chao;Ma Xiaodong;Yuan Lihua;Liu Shenquan;Zheng Kexiao;Li Jiong
提出了一种稳健的高通量悬浮阵列来分析生物分子。为了利用其核酸检测能力，我们设计了一种多重巢式不对称 PCR (MNAS PCR)，无需复杂的优化即可高效产生单链 DNA (ssDNA)。多重 HPV 基因分型具有高选择性、高灵敏度和 20 分钟内快速杂交的特点。
A robust high throughput suspension array was proposed to analyze biomolecules. To exploit its capacity for nucleic acid detection, we designed a multiplex nested asymmetric PCR (MNAS PCR) that can produce single stranded DNA (ssDNA) efficiently without complicated optimization. Multiplexed HPV genotyping was demonstrated with high selectivity, high sensitivity and rapid hybridization within 20 minutes.