winged eye Induces Transdetermination of Drosophila Imaginal Disc by Acting in Concert with a Histone Methyltransferase, Su(var)3-9
winged eye Induces Transdetermination of Drosophila Imaginal Disc by Acting in Concert with a Histone Methyltransferase, Su(var)3-9
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有翼眼通过与组蛋白甲基转移酶 Su(var)3-9 协同作用诱导果蝇成虫盘的转决定
DOI：
10.1016/j.celrep.2017.11.105
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发表时间：
2018
期刊：
影响因子：
8.8
通讯作者：
Kurata Shoichiro
中科院分区：
文献类型：
--
作者：
Masuko Keita;Fuse Naoyuki;Komaba Kanae;Katsuyama Tomonori;Nakajima Rumi;Furuhashi Hirofumi;Kurata Shoichiro
果蝇髓核细胞表现出显著的改变细胞命运的能力，这种现象被称为反式决定(Td)。我们以前认为有翼眼(Wge)是在眼成像盘过度表达时诱发眼对翼TD的一个因素，但TD背后的分子机制仍很不清楚。在这里，我们发现WGE诱导了各种组蛋白修饰，并增强了组蛋白H3(H3K9)上Lys9的甲基化，这是异染色质的一个特征。组蛋白甲基转移酶SU(Var)3-9是Ge介导的H3K9甲基化和眼对翼TD所必需的。SU(Var)3-9也是经典创伤诱导的TD所必需的，但对正常发育不是必需的，这表明它参与了几种类型的假想视盘TD。转录组分析表明，WGE独立于SU(Var)3-9抑制眼睛识别基因，并通过与SU(Var)3-9共同作用激活TD相关基因。这些发现为在细胞命运转换的渐进步骤中不同类型的染色质调节提供了新的见解。
Drosophilaimaginal disc cells exhibit a remarkable ability to convert cell fates in response to various perturbations, a phenomenon called transdetermination (TD). We previously identifiedwinged eye(wge) as a factor that induces eye-to-wing TD upon overexpression in eye imaginal discs, but the molecular mechanisms underlying TD have remained largely unclear. Here, we found thatwgeinduces various histone modifications and enhances the methylation of Lys9 on histone H3 (H3K9), a feature of heterochromatin. A histone methyltransferase, Su(var)3-9, is required forwge-mediated H3K9 methylation and eye-to-wing TD. Su(var)3-9 is also required for classical wound-induced TD but not for normal development, suggesting its involvement in several types of imaginal disc TDs. Transcriptome analysis revealed thatwgerepresses eye identity genes independently of Su(var)3-9 and activates TD-related genes by acting together with Su(var)3-9. These findings provide new insights into diverse types of chromatin regulation at progressive steps of cell-fate conversions.