Characterization of Optimal Aptamer‐Microarray Binding Chemistry and Spacer Design
Characterization of Optimal Aptamer‐Microarray Binding Chemistry and Spacer Design
复制标题
最佳适体微阵列结合化学的表征和间隔区设计
DOI：
10.1002/ceat.201000551
复制
发表时间：
2011
影响因子：
2.1
通讯作者：
T. Scheper
中科院分区：
文献类型：
--
作者：
G. Zhu;M. Lübbecke;J. Walter;F. Stahl;T. Scheper
与传统的蛋白质微阵列相比，适体微阵列是一种相对较新且具有竞争力的分析方法。三维折叠适体显示出优异的结合特异性和亲和力，因此可以用作抗体的替代品。研究了不同表面和间隔物组合对适体微阵列应用中适体的固定和结合效果。此外，间隔集成在适配体的末端位置上的自制和市售的微阵列支持的影响进行了探索。
Compared to conventional protein microarrays, the aptamer microarray is a relatively new and competitive method for analytical applications. The three-dimensional folded aptamers show excellent binding specificity and affinity, and thus, can be used as an alternative to antibodies. Immobilization and binding effects of aptamers under different combinations of surfaces and spacers for aptamer-microarray applications are investigated. In addition, the effects of spacers integrated at the terminal position of aptamers on made in-house and commercially available microarray supports are explored.
影响因子：
56.9
作者：
TUERK, C;GOLD, L
通讯作者：
GOLD, L