DEVELOPMENTAL AND TISSUE-SPECIFIC REGULATION OF MOUSE TELOMERASE AND TELOMERE LENGTH
DEVELOPMENTAL AND TISSUE-SPECIFIC REGULATION OF MOUSE TELOMERASE AND TELOMERE LENGTH
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DOI：
10.1073/pnas.92.11.4818
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发表时间：
1995-05-23
影响因子：
11.1
通讯作者：
GREIDER, CW
中科院分区：
文献类型：
--
作者：
PROWSE, KR;GREIDER, CW
人类体细胞端粒缩短和端粒酶激活与细胞永生化和细胞衰老有关。为了进一步研究端粒酶在永生化中的作用，我们分析了原代小鼠成纤维细胞、自然永生化细胞克隆和小鼠组织中的端粒长度和端粒酶活性。在原代细胞培养中，端粒长度随细胞倍增而减少，未检测到端粒酶活性。相比之下，在自然永生化的克隆中，端粒保持在稳定的长度，并且存在端粒酶活性。为了确定端粒缩短是否在体内发生，我们分析了不同年龄小鼠组织中的端粒酶和端粒长度。新生小鼠体内不同组织之间的端粒长度相似，而成年小鼠不同组织间的端粒长度不同。这些发现表明，在体内发育和衰老过程中，小鼠端粒酶存在组织特异性调节。与人类组织相比，大多数小鼠组织都有活性的端粒酶。这些组织中端粒酶的存在可能反映了原代小鼠细胞在培养中相对于人类细胞更容易永生化。
Telomere shortening and telomerase activation in human somatic cells have been implicated in cell immortalization and cellular senescence. To further study the role of telomerase in immortalization, we assayed telomere length and telomerase activity in primary mouse fibroblasts, in spontaneously immortalized cell clones, and in mouse tissues. In the primary cell cultures, telomere length de creased with increased cell doublings and telomerase activity was not detected. In contrast, in spontaneously immortalized clones, telomeres were maintained at a stable length and telomerase activity was present. To determine if telomere shortening occurs in vivo, we assayed for telomerase and telomere length in tissues from mice of different ages. Telomere length was similar among different tissues within a newborn mouse, whereas telomere length differed between tissues in an adult mouse. These findings suggest that there is tissue-specific regulation of mouse telomerase during development and aging in vivo. In contrast to human tissues, most mouse tissues had active telomerase. The presence of telomerase in these tissues may reflect the ease of immortalization of primary mouse cells relative to human cells in culture.