SARs do not impair position-dependent expression of a kit/lacZ transgene.
SARs do not impair position-dependent expression of a kit/lacZ transgene.
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SAR 不会损害 kit/lacZ 转基因的位置依赖性表达。
DOI：
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
J. Panthier
中科院分区：
文献类型：
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作者：
P. de Sepulveda;P. Salaün;N. Maas;C. André;J. Panthier
我们已经克隆和测序的小鼠c-kit启动子区的4.2 kb。对kit/lacZ融合基因的转基因胚胎的分析显示（i）在神经视网膜中的意想不到的表达，和（ii）对位置效应的不寻常的敏感性。这表明主要的c-kit组织特异性调节元件位于近端启动子之外。我们利用kit/lacZ转基因的内在特性，研究了支架附着区（SAR）在体内环境中赋予基因调控位置独立性的潜力。在转基因胚胎中没有发现kit/lacZ转基因的SAR依赖性分离，表明SAR序列缓冲侧翼序列对转基因表达的影响的能力有限。
We have cloned and sequenced 4.2 kb of the murine c-kit promoter region. Analysis of transgenic embryos for a kit/lacZ fusion gene showed (i) an unexpected expression in the neural retina, and (ii) an unusual sensitivity to position effects. This indicates that major c-kit tissue specific regulating elements lie outside the proximal promoter. We took advantage to the intrinsic properties of the kit/lacZ transgene to investigated the potential of scaffold attachment regions (SARs) to confer position-independence on gene regulation in an in vivo context. No SAR-dependent isolation of the kit/lacZ transgene was found in the transgenic embryos, indicating a limited capacity of SAR sequences to buffer the effect of flanking sequences on transgene expression.