MECHANISMS OF ALPHA-FETOPROTEIN TRANSFER IN THE PERFUSED HUMAN PLACENTAL COTYLEDON FROM UNCOMPLICATED PREGNANCY
MECHANISMS OF ALPHA-FETOPROTEIN TRANSFER IN THE PERFUSED HUMAN PLACENTAL COTYLEDON FROM UNCOMPLICATED PREGNANCY
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DOI：
10.1172/jci118277
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发表时间：
1995-11-01
影响因子：
15.9
通讯作者：
NELSON, DM
中科院分区：
文献类型：
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作者：
BROWNBILL, P;EDWARDS, D;NELSON, DM
本文通过甲胎蛋白转移量与甲胎蛋白细胞化学定位的相关性研究了甲胎蛋白跨胎盘转移的机制。胎盘子叶体外双向灌流，以含脐血浆的胎儿或母体灌流液为甲胎蛋白来源，稳态甲胎蛋白清除(校正内源性甲胎蛋白释放)在胎儿至母体方向(n=10)为0.973+/-0.292 mU/m in L/分钟，显著高于(P<；0.02)母胎方向(n=5)；0.022+/-0.013亩L/分钟每克)，清除类似大小的蛋白质，辣根过氧化物酶也是不对称的，但小示踪剂肌酸清除不对称。我们用一种单抗将AFP定位于绒毛合体滋养细胞不连续区域的纤维蛋白沉积、这些沉积中的细胞滋养细胞、一些绒毛上的合体滋养细胞和蜕膜中的滋养细胞，我们得出结论：AFP在胎盘中的转移是不对称的，AFP转移有两条可能的途径：(A)从胎儿循环进入绒毛核心，并穿过绒毛合体滋养细胞不连续处的纤维蛋白沉积进入母体循环；以及(B)蜕膜中的AFP可以进入穿过基底板的血管。
We investigated the mechanisms of alphafetoprotein (AFP) transfer across the human placenta by correlating measurements of AFP transfer with cytochemical localization of AFP, Placental cotyledons were dually perfused in vitro with either the fetal or maternal perfusate containing umbilical cord plasma as a source of AFP, Steady state AFP clearance, corrected for release of endogenous AFP, was 0.973+/-0.292 mu l/min per gram in the fetal to maternal direction (n = 10), significantly higher (P < 0.02) than that in the maternal to fetal direction (n = 5; 0.022+/-0.013 mu l/min per gram), Clearance of a similarly sized protein, horseradish peroxidase was also asymmetric but clearance of the small tracer creatinine was not. Using a monoclonal antibody, we localized AFP to fibrinoid deposits in regions of villi with discontinuities of the syncytiotrophoblast, to cytotrophoblast cells in these deposits, to syncytiotrophoblast on some villi, and to trophoblast cells in the decidua, We conclude that AFP transfer in the placenta is asymmetric and that there are two available pathways for AFP transfer: (a) from the fetal circulation into the villous core and across fibrinoid deposits at discontinuities in the villous syncytiotrophoblast to enter the maternal circulation; and (b) AFP present in the decidua could enter vessels that traverse the basal plate.