Retrograde double-labeling study of common afferent projections to the dorsal raphe and the nuclear core of the locus coeruleus in the rat
Retrograde double-labeling study of common afferent projections to the dorsal raphe and the nuclear core of the locus coeruleus in the rat
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DOI：
10.1002/cne.20365
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发表时间：
2005-01-10
影响因子：
2.5
通讯作者：
Waterhouse, BD
中科院分区：
文献类型：
--
作者：
Lee, HS;Kim, MA;Waterhouse, BD
在大鼠中缝背核（DR）和蓝斑核（LC）的核芯内成对注射金标记的和灭活的麦胚凝集素-辣根过氧化物酶（WGA-apo-HRP-gold）和荧光金（FG）逆行示踪剂，分析了DR和LC的共同传入投射。我们的结果表明，最大数量的双标记神经元位于不同的视前区，包括内侧视前区，外侧视前核，腹外侧视前核。大多数标记细胞也观察到在外侧下丘脑，标记细胞的数量是相当的内侧视前区或外侧视前核的神经元。在下丘脑的各个区域，包括前、内侧结节、后和弓状核，以及中脑区域，包括黑质和腹外侧/外侧导水管周围灰质，观察到一些双标记细胞。在前边缘/下边缘前额叶皮质、Broca斜角带、终纹床核和脑桥/延髓区（包括各种中缝核、Barrington核、前巨细胞、舌下前置核、蓝斑下核和背内侧被盖区）也观察到细胞。虽然需要进行电生理学研究，大量的双标记神经元位于视前区以及外侧下丘脑可能有其主要作用，同时控制这些单胺能核作为一种手段，影响各种睡眠和觉醒状态的动物。位于其他位置的双标记细胞可能会影响其他各种功能，如镇痛，认知和应激反应。(C)2004 Wiley-Liss，Inc.
Common afferent projections to the dorsal raphe (DR) and locus coeruleus (LC) nuclei were analyzed in the rat by making paired injections of retrograde tracers, gold-conjugated and inactivated wheatgerm agglutinin-horseradish peroxidase (WGA-apo-HRP-gold) and Fluorogold (FG), into the DR and the nuclear core of the LC. Our results demonstrate that the largest number of double-labeled neurons was located at various preoptic regions including medial preoptic area, lateral preoptic nucleus, and ventrolateral preoptic nucleus. The majority of labeled cells were also observed at the lateral hypothalamus, where the number of labeled cells was comparable to that of neurons at the medial preoptic area or lateral preoptic nucleus. A few double-labeled cells were observed at various hypothalamic regions including anterior, medial tuberal, posterior, and arcuate nuclei, as well as mesencephalic areas including substantia nigra compacta and ventrolateral/lateral periaqueductal gray matter. Cells were also observed at prelimbic/infralimbic prefrontal cortices, diagonal band of Broca, bed nucleus of stria terminalis, and pontine/medullary regions including various raphe nuclei, Barrington's nucleus, gigantocellularis, paragigantocellularis, prepositus hypoglossi, subcoeruleus, and dorsomedial tegmental area. Although electrophysiological studies need to be performed, a large number of double-labeled neurons located at preoptic regions as well as lateral hypothalamus might have their major role in simultaneous control over these monoaminergic nuclei as a means of influencing various sleep and arousal states of the animal. Double-labeled cells at the other locations might be positioned to influence a variety of other functions such as analgesia, cognition, and stress responses. (C) 2004 Wiley-Liss, Inc.