EFFECTS OF HOST PLANT DEVELOPMENT AND GENETIC-DETERMINANTS ON THE LONG-DISTANCE MOVEMENT OF CAULIFLOWER MOSAIC-VIRUS IN ARABIDOPSIS
EFFECTS OF HOST PLANT DEVELOPMENT AND GENETIC-DETERMINANTS ON THE LONG-DISTANCE MOVEMENT OF CAULIFLOWER MOSAIC-VIRUS IN ARABIDOPSIS
复制标题
DOI：
10.1105/tpc.5.2.191
复制
发表时间：
1993-02-01
期刊：
影响因子：
11.6
通讯作者：
HOWELL, SH
中科院分区：
文献类型：
--
作者：
LEISNER, SM;TURGEON, R;HOWELL, SH
在系统感染期间，病毒通过植物维管系统长距离移动。花椰菜花叶病毒（CaMV）在拟南芥中的长距离移动已经用一种称为植物骨架杂交的整株原位杂交技术进行了研究。CaMV在韧皮部的长距离移动主要是随着同化物从源叶到库叶的流动。在植物发育过程中，库源关系发生变化，CaMV可以侵入的植物区域逐渐减少。在拟南芥中，我们发现影响植物发育速率的条件显著影响CaMV的长距离运动，因为在正常条件下，植物发育速率与病毒运动的动力学密切匹配。拟南芥的早期开花的生态型和突变体表现出对系统性CaMV感染的抗性，我们称之为“发育抗性”。"发育抗性是由于早花植物的莲座叶在生命的早期就成熟了，病毒无法进入。另一方面，如果早期开花植物的发育因次优生长条件而延迟，则接种的植物似乎对病毒更敏感，并且系统感染变得更普遍。我们已经发现，其他拟南芥生态型，如Enkheim-2（En-2），显示出另一种形式的抗病毒运动，这不是基于发育或生长条件。生态型En-2的抗病毒性主要受一个位点的显性性状控制。
During systemic infections, viruses move long distances through the plant vascular system. The long-distance movement of cauliflower mosaic virus (CaMV) in Arabidopsis has been examined using a whole plant in situ hybridization technique called plant skeleton hybridization. CaMV moves long distance through the phloem largely following the flow of photoassimilates from source to sink leaves. During the course of plant development, sink-source relationships change and the region of the plant that CaMV can invade is progressively reduced. In Arabidopsis, we have found that conditions that influence the rate of plant development dramatically impact the long-distance movement of CaMV, because under normal conditions the rate of plant development is closely matched to the kinetics of virus movement. Ecotypes and mutants of Arabidopsis that flower early show a form of resistance to systemic CaMV infection, which we call ''developmental resistance.'' Developmental resistance results from the fact that the rosette leaves mature early in the life of an early flowering plant and become inaccessible to virus. On the other hand, if the development of early flowering plants is retarded by suboptimal growth conditions, inoculated plants appear more susceptible to the virus and systemic infections become more widespread. We have found that other Arabidopsis ecotypes, such as Enkheim-2 (En-2), show another form of resistance to virus movement that is not based on developmental or growth conditions. The virus resistance in ecotype En-2 is largely conditioned by a dominant trait at a single locus.