A 72-hour high fat diet increases transcript levels of the neuropeptide galanin in the dorsal hippocampus of the rat.
A 72-hour high fat diet increases transcript levels of the neuropeptide galanin in the dorsal hippocampus of the rat.
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DOI：
10.1186/s12868-015-0188-9
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发表时间：
2015-08-11
期刊：
影响因子：
2.4
通讯作者：
Baile CA
中科院分区：
文献类型：
--
作者：
Gan L;England E;Yang JY;Toulme N;Ambati S;Hartzell DL;Meagher RB;Baile CA
最近的证据表明，海马体——一种通常与学习和记忆有关的大脑结构——是调节食物摄入、肥胖发展和后果的关键。摄入高脂肪饮食（HFD）会导致消费行为改变、海马损伤和认知缺陷。虽然许多研究报告了慢性食用HFD和肥胖后HFD的影响，但很少研究急性食用HFD后发生的事件。在本研究中，雄性大鼠被喂食对照饲料（10%脂肪/千卡）或HFD（45%脂肪/千卡）72小时。72小时结束时，收集血清和组织并称重。脑分别快速冷冻或福尔马林固定，为qRT-PCR或免疫组织化学做准备。急性摄入HFD导致血清瘦素和胆固醇水平升高，最终体重或脂肪组织质量没有显著变化。大鼠海马背侧神经保护肽丙氨酸的转录显著上调，脑源性神经营养因子和组蛋白去乙酰化酶2呈增加趋势。在海马腹侧，组蛋白去乙酰化酶4显著升高，甘丙肽受体1显著降低。免疫组化结果证实了海马背侧CA1/CA2区存在强的甘丙肽。这些结果为急性HFD摄入后海马特定功能区域的明显反应提供了证据。
Recent evidence identifies the hippocampus, a brain structure commonly associated with learning and memory, as key to the regulation of food intake and the development and consequences of obesity. Intake of a high fat diet (HFD) results in altered consumptive behavior, hippocampal damage, and cognitive deficits. While many studies report the effects of HFD after chronic consumption and in the instance of obesity, few examine the events that occur following acute HFD consumption. In this study, male rats were fed either a control diet (10% fat by kcal) or HFD (45% fat by kcal) for 72 h. At the end of the 72-h period, serum and tissues were collected and weighed. Brains were rapidly frozen or formalin-fixed in preparation for qRT-PCR or immunohistochemistry, respectively. Acute intake of HFD resulted in higher serum levels of leptin and cholesterol, with no significant changes in final body weight or adipose tissue mass. In the dorsal hippocampus, transcription of the neuroprotective peptide galanin was significantly upregulated along with a trend for an increase in brain-derived neurotrophic factor and histone deacetylase 2 in the rats fed HFD. In the ventral hippocampus, there was a significant increase in histone deacetylase 4 and a decrease in galanin receptor 1 in this group. Results from immunohistochemistry validate strong presence of the galanin peptide in the CA1/CA2 region of the dorsal hippocampus. These results provide evidence for a distinct response in specific functional regions of the hippocampus following acute HFD intake.