Development of Genomic Simple Sequence Repeat Markers for Yam
Development of Genomic Simple Sequence Repeat Markers for Yam
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DOI：
10.2135/cropsci2014.10.0725
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发表时间：
2015-09
期刊：
影响因子：
2.3
通讯作者：
Muluneh Tamiru;S. Yamanaka;Chikako Mitsuoka;Pachakkil Babil;H. Takagi;A. Lopez-Montes;A. Sartie;R. Asiedu;R. Terauchi
中科院分区：
文献类型：
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作者：
Muluneh Tamiru;S. Yamanaka;Chikako Mitsuoka;Pachakkil Babil;H. Takagi;A. Lopez-Montes;A. Sartie;R. Asiedu;R. Terauchi
薯蓣属是一种主要的主食作物，因其含淀粉的块茎而被广泛种植。迄今为止，很少有标记资源可公开用作这种经济上重要的作物的遗传和基因组研究的工具。本研究从富集的黄山药基因组文库中筛选出90个简单重复序列（SSR）标记。cayenensis Lam.交叉扩增结果表明，其中85个（94.4%）和51个（56.7%）SSRs能成功地转移到两种主要栽培种中。圆叶树和D.阿拉塔属，分别地。根据DNA条带的可靠性和重复性选择30个标记，用12个D. cayenensis，48 D. rotundata和48 D.阿拉塔加入。相应地，每个位点的等位基因数在2 ~ 8个之间。cayenensis（平均3.9只），D.圆叶薯蓣（D. rotundata）2 ~ 22个，平均13.9个;阿拉塔（平均值= 12.1）。平均观察杂合度和期望杂合度分别为0.156和0.634（D. cayenensis）、0.326和0.853（D. rotundata）、0.247和0.836（D.阿拉塔）。聚类的基础上，至少有四个的五个栽培的拟南芥物种，包括D。cayenensis、D. rotundata，圆叶拟步行虫D.阿拉塔、D. dumetorum（Kunth）pax.，和D.黄珠子属，除D.杜美托仑这些新的SSR标记在遗传多样性分析和分子标记辅助育种等方面具有重要的应用价值。
Yam (Dioscorea spp.) is a major staple crop widely cultivated for its starchy tubers. To date, very few marker resources are publicly avail - able as tools for genetic and genomic studies of this economically important crop. In this study, 90 simple sequence repeat (SSr) markers were developed from an enriched genomic library of yellow Guinea yam (D. cayenensis Lam.). Cross- amplification revealed that 85 (94.4%) and 51 (56.7%) of these SSrs could be successfully transferred to the two major cultivated species of D. rotundata poir. and D. alata L., respec - tively. polymorphisms in 30 markers selected on the basis of reliability and reproducibility of DNA bands were evaluated using a panel of 12 D. cayenensis, 48 D. rotundata, and 48 D. alata accessions. Accordingly, number of alleles per locus ranged from 2 to 8 in D. cayenensis (mean = 3.9), 3 to 30 in D. rotundata (mean = 13.9), and 2 to 22 in D. alata (mean = 12.1). The average observed and expected heterozygosi- ties were 0.156 and 0.634 ( D. cayenensis), 0.326 and 0.853 (D. rotundata), and 0.247 and 0.836 (D. alata), respectively. Clustering based on six SSrs that were polymorphic in at least four of the five cultivated Dioscorea species studied, including D. cayenensis, D. rotundata, D. alata, D. dumetorum (Kunth) pax., and D. bulbifera L., detected groups consistent with the phyloge - netic relationships of the species except for D. dumetorum. These new SSr markers are invalu - able resources for applications such as genetic diversity analysis and marker-assisted breeding.