Identification and characterization of structural proteins of orchid fleck virus
Identification and characterization of structural proteins of orchid fleck virus
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DOI：
10.1007/s00705-008-0268-6
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发表时间：
2009-01-01
影响因子：
2.7
通讯作者：
Tamada, Tetsuo
中科院分区：
文献类型：
--
作者：
Kondo, Hideki;Maeda, Takanori;Tamada, Tetsuo
兰花斑点病毒(OFV)具有与植物弹状病毒序列相似的两段负义RNA基因组。OFV的无包膜弹状颗粒类似于弹状病毒的内部核糖核蛋白(RNP)-M蛋白结构，但它们的大小约为典型植物弹状病毒的一半。纯化的制剂含有完整的子弹状和丝状颗粒。丝状颗粒表现为紧密盘绕的螺旋结构或螺旋扭曲的螺旋结构，类似于弹状病毒的RNP复合体。在含有2%Triton X-100和0.8M氯化钠的溶液中，OFV子弹状颗粒结构稳定。Western印迹分析表明，子弹状颗粒含有N、P和M蛋白，而丝状颗粒主要含有N和P蛋白。此外，在两种类型的颗粒中都检测到了少量的L蛋白。因此，OFV的结构蛋白具有与弹状病毒相似的性质，不同之处在于这些颗粒是无包膜的，并且在高盐条件下相对抵抗洗涤剂处理。
Orchid fleck virus (OFV) has a bipartite negative-sense RNA genome with sequence similarities to plant rhabdoviruses. The non-enveloped bullet-shaped particles of OFV are similar to those of the internal ribonucleoprotein (RNP)-M protein structure of rhabdoviruses, but they are about half the size of typical plant rhabdoviruses. Purified preparations contained intact bullet-shaped and filamentous particles. The filamentous particles showed a tightly coiled coil structure or a coiled structure with a helical twist, which resembles the RNP complex of rhabdoviruses. OFV bullet-shaped particles were structurally stable in solutions containing 2% Triton X-100 and 0.8 M NaCl. Western blot analyses revealed that the bullet-shaped particles contained N, P and M proteins, while filamentous particles contained mainly N and P proteins. In addition, a small amount of the L protein was detected in both types of particles. Thus, the structural proteins of OFV have properties similar to those of rhabdoviruses, except that the particles are non-enveloped and are relatively resistant to detergent-treatment under high-salt conditions.