Increased IL-12 release by monocytes in nephrotic patients
Increased IL-12 release by monocytes in nephrotic patients
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DOI：
10.1046/j.1365-2249.1999.00975.x
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发表时间：
1999-08-01
影响因子：
4.6
通讯作者：
Kanmatsuse, K
中科院分区：
文献类型：
--
作者：
Matsumoto, K;Kanmatsuse, K
微小病变型肾病综合征(MCNS)的发病机制尚不清楚。据推测，这种肾小球疾病可能是一种全身性细胞免疫紊乱。IL-12是一种异源二聚体细胞因子，主要由抗原提呈细胞产生，在诱导细胞免疫中起主要作用。为了深入了解这种细胞因子的参与，我们在体外检测了临床状态稳定或活跃的MCNS和IgA肾病(IgAN)患者的IL-12水平。将这些水平与健康对照组的值进行比较。与正常对照组相比，肾病综合征(NS)患者外周血单核细胞(PBM)培养上清液中IL-12的自发释放和脂多糖刺激的释放均显著增加。此外，当对单个MCNS患者的临床病情进行跟踪时，IL-12水平在活动期升高，并在患者缓解期恢复正常。MCNS患者IL-12水平与血管通透性因子(VPF)水平呈显著正相关。本研究描述了两种类型肾病患者PBM体外释放IL-12与疾病活动性的相关性，以及与同一培养物释放VPF的相关性。这项研究有助于了解这类疾病，观察结果可能具有预后/诊断价值。
The pathogenesis of minimal-change nephrotic syndrome (MCNS) is obscure. It has been postulated that this glomerular disease may be a systemic disorder of cell-mediated immunity. IL-12 is a heterodimeric cytokine that is produced primarily by antigen-presenting cells and plays a primary role in the induction of cell-mediated immunity. To gain insight into the involvement of this cytokine, in vitro IL-12 levels were assessed in patients with MCNS and IgA nephropathy (IgAN) who were in either a stable or active clinical condition. The levels were compared with Values in healthy controls. Significantly increased spontaneous and lipopolysaccharide (LPS)-stimulated release of IL-12 was detected in peripheral blood monocyte (PBM) cultures of MCNS and IgAN patients with the nephrotic syndrome (NS) compared with those of normal controls. Moreover, when individual MCNS patients were followed through their clinical illness, IL-12 levels were increased during the active phase and normalized as the patients went into remission. The amounts of IL-12 are significantly correlated with the levels of vascular permeability factor (VPF) in MCNS patients. This study describes a correlation between in vitro IL-12 release by PBM from two types of nephrotic patients and their disease activity, as well as a correlation with release of VPF by the same cultures. The study contributes to the understanding of this class of diseases and the observations may have a prognostic/diagnostic value.