Periodic transcriptional organization of the E-coli genome
Periodic transcriptional organization of the E-coli genome
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DOI：
10.1016/j.jmb.2004.05.039
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发表时间：
2004-07-23
影响因子：
5.6
通讯作者：
Képès, F
中科院分区：
文献类型：
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作者：
Képès, F
原核生物类核内的转录组织可能取决于并决定染色体的结构。事实上，转录调节因子的免疫荧光定位已经揭示了活跃转录的大肠杆菌细胞中的病灶。此外，结构和生物化学方法表明，每个基因组有类似于50个独立的环结构域。这里我用四个E来说明。在大肠杆菌菌株中，由序列特异性转录调节子控制的基因倾向于位于编码该调节子的基因旁边，或者位于染色体长度的1/50的倍数的规则距离处。这种周期性与染色体的螺线管外组织一致，其将相互作用的伴侣、调节分子及其DNA结合位点聚集成焦点。因此，在DNA上真正的调控位点的结合将通过在其附近的共转录翻译调控因子来优化。(C)2004爱思唯尔有限公司保留所有权利。
The organization of transcription within the prokaryotic nucleoid may be expected to both depend on and determine the structure of the chromosome. Indeed, immunofluorescence localization of transcriptional regulators has revealed foci in actively transcribing Escherichia coli cells. Furthermore, structural and biochemical approaches suggest that there are similar to50 independent loop domains per genome. Here I show that in four E. coli strains, genes that are controlled by a sequence-specific transcriptional regulator tend to locate next to the gene encoding this regulator, or at regular distances that are multiples of 1/50th of the chromosome length. This periodicity is consistent with a solenoidal epi-organization of the chromosome, which would gather into foci the interacting partners; the regulator molecules and their DNA binding sites. Binding at genuine regulatory sites on DNA would thus be optimized by co-transcriptionally translating regulators in their vicinity. (C) 2004 Elsevier Ltd. All rights reserved.