Mushroom lectin enhanced immunogenicity of HBV DNA vaccine in C57BL/6 and HBsAg-transgenic mice
Mushroom lectin enhanced immunogenicity of HBV DNA vaccine in C57BL/6 and HBsAg-transgenic mice
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蘑菇凝集素增强 C57BL/6 和 HBsAg 转基因小鼠中 HBV DNA 疫苗的免疫原性
DOI：
10.1016/j.vaccine.2013.02.062
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发表时间：
2013-04-26
期刊：
影响因子：
5.5
通讯作者：
Kang, Youmin
中科院分区：
文献类型：
--
作者：
Gao, Wenjuan;Sun, Yuhan;Kang, Youmin
DNA疫苗是激活抗B型肝炎病毒（HBV）感染的免疫应答的一种有前途的策略。然而，积累的数据表明，单独的DNA疫苗接种产生弱的免疫应答。为提高HBV DNA疫苗的免疫原性，将从平菇中纯化的凝集素（POL）作为HBV DNA疫苗的佐剂，分别接种C57 BL/6和HBV表面抗原转基因（HBVsAg-Tg）小鼠。我们的数据表明，低剂量的POL（1 μ g/只）与HBV DNA疫苗联合刺激更强的HBV特异性迟发型超敏反应（DTH）和更高的HBV特异性IgG水平比高剂量的POL组（5 μ g/只和10 μ g/只）。POL在免疫的C57 BL/6和HBVsAg-Tg小鼠中激活了强烈的Th 2和Tc 1细胞应答。POL作为HBV DNA疫苗的佐剂，能有效提高HBV Tg小鼠的抗HBV表面蛋白抗体（HBVsAb）水平，降低HBVsAg水平。除Th 1、Th 2和Tc 1细胞亚型外，POL还向HBVsAg-Tg小鼠肝脏浸润了更多的淋巴细胞。这些结果表明，POL作为佐剂增强了HBV DNA疫苗的免疫原性，并有效地刺激了HBV-Ag-Tg小鼠治疗的免疫应答。我们的研究结果表明蘑菇凝集素作为HBV DNA疫苗佐剂的潜力。(c)2013爱思唯尔有限公司保留所有权利。
DNA vaccination is a promising strategy for activating immune responses against hepatitis B virus (HBV) infection. However, the accumulated data have shown that DNA vaccination alone generates weak immune responses. To enhance the immunogenicity of HBV DNA vaccine, lectin purified from pleurotus ostreatus (POL) was used as adjuvant of HBV DNA vaccine for C57BL/6 and HBV surface antigen transgenic (HBVsAg-Tg) mice. Our data demonstrate that low dose of POL (1 mu g/mouse) in conjunction with HBV DNA vaccine stimulated stronger HBV-specific delayed-type hypersensitivity (DTH) responses and higher HBV-specific IgG level than that in high dose of POL groups (5 mu g/mouse and 10 mu g/mouse). POL activated strong Th2 and Tc1 cell responses in immunized C57BL/6 and HBVsAg-Tg mice. POL as adjuvant of HBV DNA vaccine effectively enhanced HBV surface protein antibody (HBVsAb) and decreased HBVsAg level for HBV Tg mice treatment. Furthermore, POL infiltrated more lymphocytes excluding Th1, Th2 and Tc1 cell subtypes to liver of HBVsAg-Tg mice. Together, these results suggest that POL as adjuvant enhanced immunogenicity of HBV DNA vaccination and effectively stimulated immune reaponse for HBsAg-Tg mice treatment. Our findings implicate the potential of mushroom lectin as adjuvant of HBV DNA vaccine. (c) 2013 Elsevier Ltd. All rights reserved.