EFFECTS OF KAINIC ACID ON MESSENGER-RNA LEVELS OF IL-1-BETA, IL-6, TNF-ALPHA AND LIF IN THE RAT-BRAIN
EFFECTS OF KAINIC ACID ON MESSENGER-RNA LEVELS OF IL-1-BETA, IL-6, TNF-ALPHA AND LIF IN THE RAT-BRAIN
复制标题
DOI：
10.1016/s0006-291x(05)80225-6
复制
发表时间：
1991-04-30
影响因子：
3.1
通讯作者：
SATOH, M
中科院分区：
文献类型：
--
作者：
MINAMI, M;KURAISHI, Y;SATOH, M
我们采用半定量 RT-PCR 方法检测了红藻氨酸诱导的大鼠脑区几种细胞因子（例如 IL-1β、IL-6、TNFα 和 LIF）mRNA 水平的变化。注射惊厥剂量（12mg/kg i.p.）红藻氨酸后2小时，大脑皮层（CC）、丘脑（THL）和下丘脑（HT）中的IL-1βmRNA显着增加，并在注射后4小时趋于减少。注射红藻氨酸2小时后，海马(HPP)中IL-6 mRNA被微弱诱导，4小时后CC、HPP、THL和HT中IL-6 mRNA显着增加。注射后 2 小时和 4 小时，CC、HPP、纹状体 (STR)、THL 和 HT 中 TNFαmRNA 水平显着升高。 LIF mRNA 在对照大鼠的 CC 和 HPP 中明显表达，并且通过红藻氨酸处理在 CC、HPP 和 HT 中增加。这些结果表明，红藻氨酸在不同的时间过程中增加了不同大脑区域中几种细胞因子的mRNA。
We examined the kainic acid-induced changes of mRNA levels of several cytokines such as IL-1β, IL-6, TNFαand LIF in the rat brain regions using semiquantitative RT-PCR method. IL-1βmRNA was markedly increased in the cerebral cortex (CC), thalamus (THL) and hypothalamus (HT) 2 h after the injection of kainic acid in a convulsive dose (12 mg/kg i.p.), and tended to decrease 4 h after the injection. IL-6 mRNA was weakly induced in the hippocampus (HPP) 2 h after the injection of kainic acid and was markedly increased in the CC, HPP, THL, and HT at 4 h. The level of TNFαmRNA was highly elevated in the CC, HPP, striatum (STR), THL and HT at 2 and 4 h after the injection. LIF mRNA apparently expressed in the CC and HPP of control rats and was increased in the CC, HPP and HT by the treatment with kainic acid. These results indicate that mRNAs of several cytokines are increased in various brain regions with different time-courses by kainic acid.