The effect of negative air ion exposure on Escherichia coli and Pseudomonas fluorescens
The effect of negative air ion exposure on Escherichia coli and Pseudomonas fluorescens
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DOI：
10.1080/10934520801959831
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发表时间：
2008-04
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影响因子：
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通讯作者：
A. Tyagi;B. Nirala;A. Malik;Kamlesh Singh
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文献类型：
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作者：
A. Tyagi;B. Nirala;A. Malik;Kamlesh Singh
研究了空气负离子（NAI）对大肠杆菌和荧光假单胞菌的抑菌作用。适当稀释的E.将大肠杆菌和荧光假单胞菌接种于琼脂上，在密闭室中暴露于NAI（> 2 × 106 ions/cm ~ 3）。虽然在短时间暴露（15-30分钟）后没有观察到细菌杀灭，但长达4小时的长时间暴露导致E. coli和荧光假单胞菌。当平板位于NAI发生器前面时，观察到最大的杀伤力。荧光假单胞菌的年轻指数期细胞与稳定期细胞（活力降低42%）相比更具抗性（活力降低8%）。此外，与正常细胞（42%）相比，饥饿的细胞显示出较少的活力降低（33%）。细菌菌株对NAI的敏感性取决于菌株类型、它们的生理状态以及相对于源的距离/方向。尽管应用NAI降低环境微生物负荷是一个有效的选择;但可能需要延长暴露时间以控制生理上不同的细胞，如指数期细胞和/或饥饿细胞。
Antibacterial activity of negative air ions (NAI) on Escherichia coli and Pseudomonas fluorescens has been investigated. Appropriately diluted cell suspensions of E. coli and P. fluorescens were inoculated onto agar and exposed to NAI (> 2 × 106 ions/cm3) in an airtight chamber. Although no bacterial killing was observed after short exposure (15–30 min), longer exposure upto 4 hours caused 33% and 42% reduction in viability of E. coli and P. fluorescens, respectively. Maximum killing was observed when the plates were positioned in front of the NAI generator. Young exponential phase cells of P. fluorescens were more resistant (8% reduction in viability) as compared to stationary phase cells (42% reduction in viability). Also, the starved cells displayed less reduction in viability (33%) as compared to normal cells (42%). The susceptibility of bacterial strains to NAI varies depending upon the strain type, their physiological state as well as the distance/orientation with respect to the source. Although application of NAI for decreasing the ambient microbial load is a valid option; extended exposures might be required for controlling physiologically different cells such as exponential phase cells and/or starved cells.