Acoustofluidic methods in cell analysis.
Acoustofluidic methods in cell analysis.
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DOI：
10.1016/j.trac.2019.06.034
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发表时间：
2019-07
期刊：
影响因子：
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通讯作者：
Yuliang Xie;Hunter Bachman;T. Huang
中科院分区：
文献类型：
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作者：
Yuliang Xie;Hunter Bachman;T. Huang
细胞分析是生物学和医学的中心概念。在过去的二十年里，将声波与微流控相结合的声流控技术为细胞分析的创新方法的发展做出了重大贡献。声流控技术能够对细胞和限制细胞的流体进行精确操作，这些能力已被用于许多细胞分析应用。在这篇综述文章中，我们考察了声流控方法的各种应用，包括细胞成像、细胞机械分型、循环肿瘤细胞表型、临床样品制备以及细胞-细胞相互作用和细胞-环境响应的研究。我们还对这一创新方法领域的技术优势、局限性和潜在的未来方向提供了我们的观点。
Cellular analysis is a central concept for both biology and medicine. Over the past two decades, acoustofluidic technologies, which marry acoustic waves with microfluidics, have significantly contributed to the development of innovative approaches for cellular analysis. Acoustofluidic technologies enable precise manipulations of cells and the fluids that confine them, and these capabilities have been utilized in many cell analysis applications. In this review article, we examine various applications where acoustofluidic methods have been implemented, including cell imaging, cell mechanotyping, circulating tumor cell phenotyping, sample preparation in clinics, and investigation of cell-cell interactions and cell-environment responses. We also provide our perspectives on the technological advantages, limitations, and potential future directions for this innovative field of methods.