Simultaneous Determination of Three Synthetic Glucocorticoids by Differential Pulse Stripping Voltammetry with the Aid of Chemometrics
Simultaneous Determination of Three Synthetic Glucocorticoids by Differential Pulse Stripping Voltammetry with the Aid of Chemometrics
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DOI：
10.1080/00032710802208708
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发表时间：
2008-07
影响因子：
2
通讯作者：
Y. Ni;Shuzhen Li;S. Kokot
中科院分区：
文献类型：
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作者：
Y. Ni;Shuzhen Li;S. Kokot
摘要建立了同时测定强的松、强的松和地塞米松糖皮质激素残留量的伏安法。这三种化合物在布里顿-罗宾逊缓冲液（pH 3.78）的汞电极上被还原，并观察到明确的伏安波。然而，这三种化合物的伏安图重叠严重，呈现非线性特征，因此很难单独分析它们的混合物。本文采用主成分回归（PCR）和偏最小二乘（PLS）两种化学计量学方法对重叠的伏安图进行了解析，并建立了同时测定这些化合物的校准模型。在最佳实验条件下，强的松、强的松和地塞米松的检出限（LOD）分别为5.6、8.3和16.8µg l−1。该方法也适用于兔血浆和人尿中糖皮质激素残留的测定，结果令人满意。
Abstract A novel voltammetric method for simultaneous determination of the glucocorticoid residues prednisone, prednisolone, and dexamethasone was developed. All three compounds were reduced at a mercury electrode in a Britton–Robinson buffer (pH 3.78), and well-defined voltammetric waves were observed. However, the voltammograms of these three compounds overlapped seriously and showed nonlinear character, and thus, it was difficult to analyze the compounds individually in their mixtures. In this work, two chemometrics methods, principal component regression (PCR) and partial least squares (PLS), were applied to resolve the overlapped voltammograms, and the calibration models were established for simultaneous determination of these compounds. Under the optimum experimental conditions, the limits of detection (LOD) were 5.6, 8.3, and 16.8 µg l−1 for prednisone, prednisolone, and dexamethasone, respectively. The proposed method was also applied for the determination of these glucocorticoid residues in the rabbit plasma and human urine samples with satisfactory results.