Studies on the Antidiabetic and Antinephritic Activities of Paecilomyces hepiali Water Extract in Diet-Streptozotocin-Induced Diabetic Sprague Dawley Rats.
Studies on the Antidiabetic and Antinephritic Activities of Paecilomyces hepiali Water Extract in Diet-Streptozotocin-Induced Diabetic Sprague Dawley Rats.
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DOI：
10.1155/2016/4368380
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发表时间：
2016
影响因子：
4.3
通讯作者：
Wang D
中科院分区：
文献类型：
--
作者：
Wang J;Teng L;Liu Y;Hu W;Chen W;Hu X;Wang Y;Wang D
蝙蝠蛾拟青霉是一种广泛应用于亚洲国家的真菌，具有多种潜在的药理活性。本研究旨在评价蝙蝠蛾拟青霉菌丝体水提取物（PHC）对高脂饮食8周后尾静脉注射链脲佐菌素（STZ）1周的糖尿病大鼠的降糖和抗肾炎作用。以0.08、0.4和2.0 g/kg剂量给予0.12 g/kg二甲双胍和PHC 4周后，观察到糖尿病大鼠体重增加、血糖降低、总胆固醇和低密度脂蛋白胆固醇水平降低。PHC通过增强胰岛素、丙酮酸激酶活性和增加糖原合成来促进葡萄糖代谢。PHC使肾脏中超氧化物歧化酶、甲烷二羧酸醛和谷胱甘肽过氧化物酶的紊乱水平恢复正常。通过抑制血清和肾脏中IL-2、IL-6、IL-10和TNF-α的水平，揭示了PHC对糖尿病肾病的保护作用。与未治疗的糖尿病大鼠相比，4周的PHC治疗导致肾脏核因子κ B表达减少。这些结果表明蝙蝠蛾拟青霉具有抗糖尿病和抗肾炎的作用，其作用机制与调节核因子κ B活性有关。
Paecilomyces hepiali is a fungus widely used in Asian countries for various potential pharmacological activities. The present study aims to evaluate the antidiabetic and antinephritic effects of the Paecilomyces hepiali mycelium water extract (PHC) in diabetic rat, which is established by eight-week high-fat diet administration followed by one-week tail intravenous injection of 25 mg/kg streptozotocin (STZ). After four-week 0.12 g/kg metformin and PHC at doses of 0.08, 0.4, and 2.0 g/kg treatment, an increment of body weight, a decrement of plasma glucose, low levels of total cholesterol, and low density lipoprotein cholesterol in diabetic rats were observed. PHC promotes glucose metabolism by enhancing insulin, pyruvate kinase activity, and increasing the synthesis of glycogen. PHC normalized the disturbed levels of superoxide dismutase, methane dicarboxylic aldehyde, and glutathione peroxidase in kidney. The inhibitory effects on the levels of interleukin-2, interleukin-6, interleukin-10, and tumor necrosis factor-α in serum and kidney revealed the protection of PHC against diabetic nephropathy. Compared with nontreated diabetic rats, four-week PHC treatment resulted in a decrement on nuclear factor kappa B expression in kidney. These results show that Paecilomyces hepiali possesses antidiabetic and antinephritic effects which are related to the modulation of nuclear factor kappa B activity.