CLONAL NATURE OF THE IMMUNE RESPONSE TO PHOSPHORYLCHOLINE
CLONAL NATURE OF THE IMMUNE RESPONSE TO PHOSPHORYLCHOLINE
复制标题
对磷酸胆碱的免疫反应的克隆性质
DOI：
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发表时间：
1974
影响因子：
15.3
通讯作者：
Joseph M. Davie
中科院分区：
文献类型：
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作者：
J. Claflin;Joseph M. Davie
描述了小鼠抗PC抗体上的一个新的独特型决定簇。通过用HOPC 8（一种PC结合骨髓瘤蛋白）免疫家兔，产生针对决定簇的抗体，并通过用PC洗脱从HOPC 8免疫吸附剂中分离抗体。这些抗体与在所有15个测试的近交系小鼠品系中产生的抗PC抗体上的结合位点决定簇反应，而不管组织相容性或同种异型，但不与其他特异性的抗体或与在其他啮齿动物物种中产生的抗PC抗体反应。这些结果与其他研究密切相关，这些研究显示在17种不同品系的小鼠中产生的抗PC抗体具有相似的结合特异性。位点相关独特型决定簇与小鼠抗HOPC 8抗血清检测到的明显不同。后一种决定簇仅存在于少数小鼠品系的抗PC抗体上，并且可能不在结合位点。
A new idiotypic determinant(s) on mouse anti-PC antibodies is described. Antibodies to the determinant(s) were raised in rabbits by immunization with HOPC 8, a PC-binding myeloma protein, and were isolated from HOPC 8 immunoadsorbent by elution with PC. These antibodies react with binding site determinants on anti-PC antibodies raised in all 15 inbred mouse strains tested regardless of histocompatibility or allotype, but fail to react with antibodies of other specificities or with anti-PC antibodies raised in other rodent species. These results correlate closely with other studies which show similar binding specificity of anti-PC antibodies raised in 17 different strains of mice. The site-associated idiotypic determinant(s) is clearly distinct from that detected by mouse anti-HOPC 8 antisera. This latter determinant(s) is present on anti-PC antibodies of only a few strains of mice and may not be in the binding site.