Lack of specificity of antibodies directed against human beta-adrenergic receptors
Lack of specificity of antibodies directed against human beta-adrenergic receptors
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DOI：
10.1007/s00210-009-0392-1
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发表时间：
2009-04-01
影响因子：
3.6
通讯作者：
van der Velden, Jolanda
中科院分区：
文献类型：
--
作者：
Hamdani, Nazha;van der Velden, Jolanda
本研究旨在探讨β-肾上腺素能受体（β AR）抗体是否可用于测定β AR在人心肌中的表达。用Western印迹法检测人左心室组织中抗β（1）AR和β（2）AR抗体的特异性。对来自特发性扩张型心肌病和缺血性心脏病患者的心脏组织和非衰竭供体进行了比较。针对β（1）AR和β（2）AR的抗体识别不同分子量的几条蛋白质条带。此外，两种抗体还识别中国仓鼠卵巢细胞中表达β（1）AR、β（2）AR甚至β（3）AR的多种蛋白质。β AR抗体不是特异性的，不适合于研究β AR在人心肌中的表达。
The present study was designed to investigate if antibodies against beta-adrenergic receptors (beta ARs) can be used to determine expression of beta AR in human myocardium. Western blotting was performed to investigate the specificity of antibodies directed against beta(1)AR and beta(2)AR in human left ventricular tissue. A comparison was made between cardiac tissue from patients with idiopathic dilated cardiomyopathy and ischemic heart disease and nonfailing donors. The antibodies directed against beta(1)AR and beta(2)AR recognized several protein bands at different molecular weights. Moreover, both antibodies also recognized multiple proteins in Chinese hamster ovary cells expressing beta(1)AR, beta(2)AR, and even beta(3)AR. beta AR antibodies are not specific and are not suited to study expression of beta AR in human myocardium.