Isolation and characterization of the unicellular diazotrophic cyanobacterium Group C TW3 from the tropical western Pacific Ocean.
Isolation and characterization of the unicellular diazotrophic cyanobacterium Group C TW3 from the tropical western Pacific Ocean.
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DOI：
10.1111/j.1462-2920.2011.02606.x
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发表时间：
2012-03
影响因子：
5.1
通讯作者：
Y. Taniuchi;Y. Chen;Houng-Yung Chen;M. Tsai;K. Ohki
中科院分区：
文献类型：
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作者：
Y. Taniuchi;Y. Chen;Houng-Yung Chen;M. Tsai;K. Ohki
从西太平洋贫营养的黑潮中分离到一株C群单细胞重氮蓝细菌，命名为TW 3。据我们所知，这是第一次成功的实验室培养的C组单细胞固氮生物从海洋水。TW 3细胞为绿色杆状，宽2.5-3.0 µm，长4.0-6.0 µm。16 SrRNA和nifH基因片段的系统发育分析表明，TW 3序列与先前分离的蓝杆藻属ATCC 51142和粘球藻属Gloeocapsa sp.的同源性超过98%，这表明TW 3是C组单细胞固氮菌的成员。此外，TW 3和蓝杆藻ATCC 51142都具有共同的形态特征;两种菌株都是无鞘和杆状的，在一个平面上显示二分裂，并具有分散的类囊体。TW 3在氮缺乏的人工海水中有氧生长，在30°C和140 µmol m（-2）s（-1）的光照强度下培养时，表现出最高的生长速率0.035 h（-1）。在12 h/12 h光暗循环条件下，固氮速率为7.31 × 10（-15）mol N cell（-1）day（-1）。利用束毛藻NIBB 1067固氮酶抗血清的免疫细胞化学染色显示，存在的重氮营养细胞共享TW 3的形态特征，从黑潮水体中分离出TW 3。
A unicellular diazotrophic cyanobacterium strain of Group C, designated TW3, was isolated from the oligotrophic Kuroshio Current of the western Pacific Ocean. To our knowledge, this represents the first successful laboratory culture of a Group C unicellular diazotroph from oceanic water. TW3 cells are green rods, 2.5-3.0 µm in width and 4.0-6.0 µm in length. Phylogenetic analyses of both 16S rRNA and nifH gene fragments indicated that the TW3 sequences were over 98% identical to those of the previously isolated Cyanothece sp. ATCC51142 and Gloeocapsa sp., suggesting that TW3 is a member of the Group C unicellular diazotrophs. In addition, both TW3 and Cyanothece sp. ATCC51142 share morphological characteristics; both strains are sheathless and rod-shaped, display binary fission in a single plane, and possess dispersed thylakoids. TW3 grows aerobically in nitrogen-deficient artificial seawater, and exhibited the highest observed growth rate of 0.035 h(-1) when cultured at 30°C and 140 µmol m(-2) s(-1) of light intensity. The nitrogen fixation rate, when grown optimally using a 12 h/12 h light-dark cycle, was 7.31 × 10(-15) mol N cell(-1) day(-1) . Immunocytochemical staining using Trichodesmium sp. NIBB1067 nitrogenase antiserum revealed the existence of diazotrophic cells sharing morphological characteristics of TW3 in the Kuroshio water from which TW3 was isolated.