Aquaporin8 regulates cellular development and reactive oxygen species production, a critical component of virulence in Botrytis cinerea
Aquaporin8 regulates cellular development and reactive oxygen species production, a critical component of virulence in Botrytis cinerea
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Aquaporin8 调节细胞发育和活性氧产生，这是灰霉病菌毒力的关键组成部分
DOI：
10.1111/nph.13721
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发表时间：
2016-03-01
期刊：
影响因子：
9.4
通讯作者：
Tian, Shiping
中科院分区：
文献类型：
--
作者：
An, Bang;Li, Boqiang;Tian, Shiping
水通道蛋白（Aquaporins，AQP）普遍存在于几乎所有生物体中，介导水选择性快速通过生物膜。水通道蛋白在植物病原真菌中的作用还不清楚，灰葡萄孢中的水通道蛋白基因同源物已经被鉴定并敲除。研究了水通道蛋白对菌丝生长和分生孢子形成、侵染结构形成以及对植物寄主毒力的影响。进一步确定了AQP8在活性氧（ROS）产生、分布和转运中的作用，在8种AQP8中，只有AQP8对B的能力是必需的。cinerea感染植物。AQP8是一种内源性质膜蛋白，可能作为一个通道，介导过氧化氢的摄取。B中AQP8的缺失。灰霉病菌完全抑制分生孢子和侵染结构的发育，并显著影响noxR的表达。进一步的观察表明，AQP8和noxR都影响了B菌丝顶端的ROS分布。灰叶此外，AQP8影响线粒体蛋白NQO1的表达。NQO1基因敲除突变体的致病力降低，这些数据有助于我们更好地理解AQP8在植物病原菌发育和致病中的重要作用。
Aquaporins (AQPs) are ubiquitous in nearly all organisms, mediating selective and rapid flux of water across biological membranes. The role of AQPs in phytopathogenic fungi is poorly understood.Orthologs of AQP genes in Botrytis cinerea were identified and knocked out. The effects of AQPs on hyphal growth and conidiation, formation of infection structures and virulence on plant hosts were examined. The role of AQP8 in reactive oxygen species (ROS) production, distribution and transport were further determined.Among eight AQPs, only AQP8 was essential for the ability of B. cinerea to infect plants. AQP8 was demonstrated to be an intrinsic plasma membrane protein, which may function as a channel and mediate hydrogen peroxide uptake. Deletion of AQP8 in B. cinerea completely inhibited the development of conidia and infection structures, and significantly affected noxR expression. Further observations revealed that both AQP8 and noxR impacted ROS distribution in the hyphal tips of B. cinerea. Moreover, AQP8 affected the expression of a mitochondrial protein, NQO1. A knockout mutant of NQO1 was observed to display reduced virulence.These data lead to a better understanding of the important role of AQP8 in the development and pathogenesis of plant pathogens.