Screening and identification of the antibacterial bioactive compounds from Lonicera japonica Thunb. leaves
Screening and identification of the antibacterial bioactive compounds from Lonicera japonica Thunb. leaves
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金银花抗菌活性成分的筛选与鉴定
DOI：
10.1016/j.foodchem.2012.10.127
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发表时间：
2013-05-01
期刊：
影响因子：
8.8
通讯作者：
Luo, Qiushui
中科院分区：
文献类型：
--
作者：
Xiong, Jianhua;Li, Shichuan;Luo, Qiushui
本研究的目的是从紫菀叶中筛选抗菌活性成分。以金黄色葡萄球菌和大肠杆菌为指示菌。抑菌试验指导的提取和逐步分区的样品产生了五个感兴趣的化合物。采用纸片扩散法测定化合物的抗菌活性。结果表明，金银花叶提取物、组分和化合物对供试菌株均有一定的抑菌活性，其中J3B2的抑菌活性最强，主要含有3，5-二-O-咖啡酰奎宁酸和4，5-二-O-咖啡酰奎宁酸。同时分离得到5个抑菌活性成分：3-O-咖啡酰奎尼酸、裂叶马钱素、木犀草苷、3，5-二-O-咖啡酰奎尼酸和4，5-二-O-咖啡酰奎尼酸，其中裂叶马钱素为首次从叶中分离得到。化合物的抑菌活性依次为：3，5-双-O-咖啡酰奎宁酸、4，5-双-O-咖啡酰奎宁酸、木犀草苷> 3-O-咖啡酰奎宁酸>裂叶马钱苷。结果表明，酚类化合物可能对L. coli的抑菌活性有重要贡献，是L. coli抑菌活性的主要来源。粳稻叶(C)2012爱思唯尔有限公司保留所有权利。
Our aim was to screen for antibacterial bioactive compounds from Lonicera japonica leaves. Staphylococcus aureus and Escherichia coli were used as the indicator bacteria. Bacteriostatic assay-guided extraction and stepwise partitioning of the samples yielded five compounds of interest. Antimicrobial activities of the compounds were determined using a disk diffusion assay. Extracts, fractions, and compounds from L japonica leaves possessed considerable antibacterial activities against the tested bacterial strains and the most active fraction was attributed to J3B2, which primarily contained 3,5-di-O-caffeoylquinic acid and 4,5-di-O-caffeoylquinic acid. Meanwhile, five bacteriostatic constituents were isolated (3-O-caffeoylquinic acid, secoxyloganin, luteoloside, 3,5-di-O-caffeoylquinic acid and 4,5-di-O-caffeoylquinic acid), among which, secoxyloganin was isolated for the first time from leaves. The antibacterial activity of the compounds was in the order of 3,5-bis-O-caffeoyl quinic acid, 4,5-bis-O-caffeoylquinic acid, luteoloside > 3-O-caffeoylquinic acid > secoxyloganin. Our results suggested that the phenolic compounds might significantly contribute to antibacterial activity and were the most responsible for the bacteriostatic activity of L. japonica leaves. (C) 2012 Elsevier Ltd. All rights reserved.