Effects of the estrous cycle on local humoral immune responses and protection of intranasally immunized female mice against herpes simplex virus type 2 infection in the genital tract
Effects of the estrous cycle on local humoral immune responses and protection of intranasally immunized female mice against herpes simplex virus type 2 infection in the genital tract
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DOI：
10.1006/viro.1996.0555
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发表时间：
1996-10-15
期刊：
影响因子：
3.7
通讯作者：
Rosenthal, KL
中科院分区：
文献类型：
--
作者：
Gallichan, WS;Rosenthal, KL
该研究表明，鼻内（i. n.）与表达单纯疱疹病毒（HSV）糖蛋白B（AdgB 8）的重组腺病毒载体之间的相互作用呈相反变化，并且依赖于发情周期的阶段。阴道冲洗液中的抗gB伊加滴度在发情期显著高于发情间期或发情前期，而特异性IgG滴度在发情间期显著高于发情期。这在孕酮处理的小鼠中得到进一步证实，其中孕酮给药诱导了间情期样状态，导致特异性IgG与伊加比率升高。有趣的是，未免疫的小鼠仅在发情间期对HSV-2的阴道内（ivag）感染敏感。小鼠i.n.免疫给予AdgB 8和孕酮的小鼠受到保护，免受致命的阴道内HSV-2攻击，尽管攻击后4天病毒复制存在。此外，大量的gB特异性伊加和IgG抗体分泌细胞存在于i.n. ivag HSV-2攻击后6天免疫的但不是未免疫的小鼠。这些结果表明，女性生殖道中特异性抗体的水平取决于发情周期的阶段。此外，I.N. AdgB 8免疫提供了显着水平的保护和特定的伊加和IgG抗体分泌细胞在生殖器组织中的IVAG感染HSV-2的决议期间。(C)出版社：Academic Press，Inc.
This study demonstrates that the levels of gB-specific IgG and IgA in vaginal washes of mice immunized intranasally (i.n.) with a recombinant adenovirus vector expressing herpes simplex virus (HSV) glycoprotein B (AdgB8) vary inversely with each other and are dependent on the stage of the estrous cycle. Anti-gB IgA titers in vaginal washes were significantly higher during estrus than diestrus or proestrus, whereas specific IgG titers were significantly higher during diestrus than estrus. This was further demonstrated in hormone-treated mice, where progesterone administration induced a diestrus-like state that resulted in elevated specific IgG-to-IgA ratios. Interestingly, unimmunized mice were only susceptible to intravaginal (ivag) infection with HSV-2 during diestrus. Mice immunized i.n. with AdgB8 and given progesterone were protected from a lethal intravaginal HSV-2 challenge, despite the fact that virus replication was present for 4 days postchallenge. Further, high numbers of gB-specific IgA and IgG antibody-secreting cells were present in both the genital tracts and the draining iliac lymph nodes of i.n.-immunized, but not unimmunized, mice 6 days following ivag HSV-2 challenge. These results demonstrate that the levels of specific antibodies in the female genital tract are dependent on the stage of the estrous cycle. Furthermore, i.n. AdgB8 immunization provided a significant level of protection and specific IgA and IgG antibody-secreting cells in the genital tissues during resolution of an ivag infection with HSV-2. (C) 1996 Academic Press, Inc.